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Contents 1 Team Cinnamaldehyde & Vanillin 1.1 June 28th 1.1.1 Transformation of 4-coumarate-CoA Ligase (4CL) and caffeic acid-O-methyl transferase (COMT) 1.1.2 Rhodobacter sphaeroides Innoculation 1.2 July 4th

Team Cinnamaldehyde & Vanillin

June 28th

Transformation of 4-coumarate-CoA Ligase (4CL) and caffeic acid-O-methyl transferase (COMT)

Experimenter: Joe

Aim: Transform biobricks 4CL ( 2013 Distribution Kit, Plate 2, Well 17P) and COMT (2013 Distribution Kit, Plate 5, Well 19D) for miniprep and later amplification through PCR

Results: Transformation done according transformation procedures. Colonies grown overnight at 37C.

Rhodobacter sphaeroides Innoculation

Experimenter: Joe

Aim: A colony of Rhodobacter sphaeroides innoculated in 5mL LB culture for DNA extraction

July 4th