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Team:USTC CHINA/Notebook/Protocols
From 2013.igem.org
Home
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Overview
Background
Design
Results
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Notebook
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Protocols
Modeling
Kill Switch
B.Subtilis Culture
Designs of Immune Experiments
Human Practice
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Protocols
Gene Clone
Gel Extraction
DNA digestion
Single Digestion
Double Digestion
Colony PCR
PCR
Ligation
Plasmid mini-prep
Transformation
The transformation of
E.coli
The transformation of
Bacillus subtilis
Overlap PCR
Extracting plasmids
Extracting plasmids from gram-positive bacterium
Extracting plasmids from gram-negative bacterium
Expression of proteins
Expression of proteins in
Bacillus subtilis
Expression of proteins in
Escherichia coli
Concentrating proteins
Transdermal experiments
Immunity&Tests
ELISA
Sample analysis
Medium
LB media
10*S base
HS media
LS media
2*YT media
Measurement
Constitutive promoter measurements
Plac promoter response to IPTG or Glucose
Notebook
Timeline
Protocols
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