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Team:Paris Bettencourt/Project/Trojan Horse
From 2013.igem.org
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SABOTAGE
Overview
Objectives = make an antibiotic resistant population sensitive to antibiotics
Design a silencing device
Design a genetic element that spread in a population
Design
A silencing system : sRNA
A vector : Phage
A non counter selected system Phagemid system
Sequential method to kill
Results
Characterization of the phagemid system
Experimental set up Results
Sequential killing
Experimental set up Results
Generalization: results for Kan
Origin of resistance
Conclusion and perspectives
Several sRNA on the same plasmid :
Building a library of sRNA :
Possible Applications :
Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
team2013@igem-paris.org
+33 1 44 41 25 22/25
team2013@igem-paris.org 
