Notebook : July 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017

Zhou

Protocol : Bacterial transformation

2 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.

B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2

Zhou

Used quantities :

• Glycerol 85% : 425
• Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BBa_K1155002 and BphR2

Anaïs

We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.