12/08/13

From 2013.igem.org

(Difference between revisions)

Latest revision as of 14:18, 13 August 2013

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Sonicating herring sperm DNA

Pipetting out sonicated samples every 30mins
- Pipette 4ul of DNA into Son 1-6 every 30mins
  - Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
  - Vortex each dilution thoroughly and centrifuge briefly.
Keep tubes on ice

Running an agarose gel for sonication analysis

To tubes 1-6, add 14 ul of sterile water and 5 ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
Use 5 ul of 1kb marker on both ends
Add buffer so that wells in the tray and the tray itself are covered
Run at 80 V for an hour and a half (gel is 1.2%)

@@ Line 1: / Line 1: @@
+<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
+{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
+!align="center"|[[Team:Leicester|Home]]
+!align="center"|[[Team:Leicester/Team|Team]]
+!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
+!align="center"|[[Team:Leicester/Project|Project]]
+!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Leicester/Modeling|Modeling]]
+!align="center"|[[Team:Leicester/Notebook|Notebook]]
+!align="center"|[[Team:Leicester/Safety|Safety]]
+!align="center"|[[Team:Leicester/Attributions|Attributions]]
+|}
+</div>
 ==Sonicating herring sperm DNA==
 *Pipetting out sonicated samples every 30mins
@@ Line 7: / Line 21: @@
 ==Running an agarose gel for sonication analysis==
-*To tubes 10xdil 1-6, add 5ul of sterile water and 5ul of orange G dye. Centrifuge briefly. Keep on ice until loading.
+*To tubes 1-6, add 14 ul of sterile water and 5 ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
-*Use 5ul of 1kb marker on one end, and 5ul of 100kb marker on other end.
+*Use 5 ul of 1kb marker on both ends
 *Add buffer so that wells in the tray and the tray itself are covered
-*Run at 100V for an hour
+*Run at 80 V for an hour and a half (gel is 1.2%)
+[[File:herringsperm_sonicate_130813.jpg]]