18/09/13

From 2013.igem.org

(Difference between revisions)
(Created page with "==Making and running an agarose gel== *Making a 3% agarose gel to run Tod PCR fusion genes from 17/09 *This is to determine that the digestion works *Running the gel with digeste...")
(Plating out TOD operon bacteria)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
 +
<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
 +
{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
 +
!align="center"|[[Team:Leicester|Home]]
 +
!align="center"|[[Team:Leicester/Team|Team]]
 +
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
 +
!align="center"|[[Team:Leicester/Project|Project]]
 +
!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
 +
!align="center"|[[Team:Leicester/Modeling|Modeling]]
 +
!align="center"|[[Team:Leicester/Notebook|Notebook]]
 +
!align="center"|[[Team:Leicester/Safety|Safety]]
 +
!align="center"|[[Team:Leicester/Attributions|Attributions]]
 +
|}
 +
</div>
 +
 +
==Making and running an agarose gel==
==Making and running an agarose gel==
*Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
*Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
Line 5: Line 20:
*Digested samples were taken from the digestions the Tod genes from previous day
*Digested samples were taken from the digestions the Tod genes from previous day
*100ng of both samples were run against a 100kb ladder
*100ng of both samples were run against a 100kb ladder
 +
 +
 +
[[File:morninggel_180913.jpg]]
 +
 +
 +
Rows are as follow:100bp ladder, undigested x, digested x, undigested b, digested b, undigested f, digested f
 +
 +
==Plating out TOD operon bacteria==
 +
*For selection of individual colonies

Latest revision as of 09:59, 20 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Making and running an agarose gel

  • Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
  • This is to determine that the digestion works
  • Running the gel with digested and undigested samples to observe the difference
  • Digested samples were taken from the digestions the Tod genes from previous day
  • 100ng of both samples were run against a 100kb ladder


Morninggel 180913.jpg


Rows are as follow:100bp ladder, undigested x, digested x, undigested b, digested b, undigested f, digested f

Plating out TOD operon bacteria

  • For selection of individual colonies