18/09/13

From 2013.igem.org

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(Plating out TOD operon bacteria)
 
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==Making and running an agarose gel==
==Making and running an agarose gel==
*Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
*Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
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==Plating out TOD operon bacteria==
==Plating out TOD operon bacteria==
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*For selection of individual colonies

Latest revision as of 09:59, 20 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Making and running an agarose gel

  • Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
  • This is to determine that the digestion works
  • Running the gel with digested and undigested samples to observe the difference
  • Digested samples were taken from the digestions the Tod genes from previous day
  • 100ng of both samples were run against a 100kb ladder


Morninggel 180913.jpg


Rows are as follow:100bp ladder, undigested x, digested x, undigested b, digested b, undigested f, digested f

Plating out TOD operon bacteria

  • For selection of individual colonies