User contributions
From 2013.igem.org
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- 12:07, 15 August 2013 (diff | hist) N Exeter/12 August 2013 (Created page with "Mini Preps of liquid cultures that worked ==NanoDrop== {| class="wikitable" |- ! Culture !! NanoDrop concentration (ng/ul) !! ul to get 250ng |- | AMP plasmid || 60.0 || 4.17...")
- 11:07, 15 August 2013 (diff | hist) Exeter/11 August 2013 (→Liquid cultures)
- 10:43, 15 August 2013 (diff | hist) N Exeter/11 August 2013 (Created page with "==Liquid cultures== * Yellow (K592010) * B0015 * RFP control * RBS + cph8 * K608002 * Magenta * OmpF * RBS + Yellow (S05058) * DH5α 1+4A * DH5α 1+4B * XL1 1+4A * X...")
- 10:21, 15 August 2013 (diff | hist) N Exeter/9 August 2013 (Created page with "==Digestions of CMY== {| class="wikitable" |- ! Culture !! NanoDrop (ng/ul) !! ul to get 250ng |- | Magenta K592012 || 106.9 || 2.34 |- | Yellow K592010 || 57.1 || 4.38 |- | Yel...")
- 09:46, 15 August 2013 (diff | hist) N Exeter/8 August 2013 (Created page with "== Liquid Cultures, Miniprepped == * K592012 (Magenta) CAM * RFP control CAM * R0082 (OmpF promoter) CAM * DH5α 3 (Tom and Adam's control) TET * DH5α 5 (Tom and Adam's RB...")
- 09:21, 15 August 2013 (diff | hist) Exeter/6 August 2013 (→Results of Transformation)
- 09:20, 15 August 2013 (diff | hist) Exeter/7 August 2013
- 09:20, 15 August 2013 (diff | hist) Exeter/7 August 2013
- 09:09, 15 August 2013 (diff | hist) N Exeter/6 August 2013 (Created page with "==Results of Transformation== 1 NEB = 11 colonies 3 NEB = 40 colonies 4 NEB = 2 colonies 2 NEB = 0 colonies 5 NEB = 0 colonies 1-5 DH5α = 0 colonies 1 NEB - RBS + cph...")
- 10:08, 8 August 2013 (diff | hist) Exeter/1 August 2013 (→Group 2)
- 10:06, 8 August 2013 (diff | hist) Exeter/31 July 2013 (→Digest)
- 10:01, 8 August 2013 (diff | hist) Exeter/25 July 2013 (→Transformations)
- 09:42, 8 August 2013 (diff | hist) Exeter/17 July 2013 (→Gel Purification Protocol)
- 09:35, 8 August 2013 (diff | hist) Exeter/17 July 2013 (→SureClean protocol)
- 09:30, 8 August 2013 (diff | hist) Exeter/16 July 2013 (→Preparing to reattempt the ligations and digestions)
- 09:30, 8 August 2013 (diff | hist) Exeter/16 July 2013 (→Preparing to reattempt the ligations and digestions=)
- 09:27, 8 August 2013 (diff | hist) Exeter/16 July 2013 (→So what went wrong with our digestion/ligation/tranformation?)
- 15:19, 7 August 2013 (diff | hist) Exeter/9 July 2013 (→Afternoon)
- 15:07, 7 August 2013 (diff | hist) Exeter/8 July 2013 (→MiniPrep plasmid extraction)
- 15:05, 7 August 2013 (diff | hist) Exeter/7 July 2013 (→Making standard liquid cultures of our transformed cells)
- 15:04, 7 August 2013 (diff | hist) Exeter/7 July 2013 (→Making standard liquid cultures of our transformed cells)
- 15:02, 7 August 2013 (diff | hist) Exeter/4 July 2013 (→(Next day, results of transformation))
- 14:45, 7 August 2013 (diff | hist) Exeter/3 July 2013 (→Making stock solutions of chloramphenicol, ampicillin and kanamycin)
- 14:39, 7 August 2013 (diff | hist) Exeter/2 July 2013 (→Make 20% weight by volume glucose solution)
- 14:37, 7 August 2013 (diff | hist) Exeter/2 July 2013 (→Learn how to make SOB growth media)
- 14:36, 7 August 2013 (diff | hist) Exeter/2 July 2013 (→Learn how to make LB broth and agar)
- 14:33, 7 August 2013 (diff | hist) Exeter/1 July 2013
- 13:39, 6 August 2013 (diff | hist) N Exeter/5 August 2013 (Created page with "=== Glycerol stocks === We made up 3 replicates of: K592018 S05058 B0015 === Nano Drop === {| class="wikitable" |- ! Culture !! NanoDrop concentration (ng/ul) !! size |-...")
- 13:27, 6 August 2013 (diff | hist) Exeter/4 August 2013 (→Liquid cultures)
- 13:27, 6 August 2013 (diff | hist) N Exeter/4 August 2013 (Created page with "=== Liquid cultures === K592018 B0015 S05058 RFP control These are all CAM")
- 13:25, 6 August 2013 (diff | hist) Exeter/1 August 2013
- 12:45, 6 August 2013 (diff | hist) N Exeter/1 August 2013 (Created page with "=== Miniprep === B0015 K592018 S05058 === Nanodrop ===")
- 12:38, 6 August 2013 (diff | hist) Exeter/31 July 2013
- 12:20, 6 August 2013 (diff | hist) Exeter/31 July 2013
- 11:43, 6 August 2013 (diff | hist) Exeter/29 July 2013 (→Digest)
- 11:43, 6 August 2013 (diff | hist) Exeter/29 July 2013 (→Liquid cultures)
- 11:43, 6 August 2013 (diff | hist) N Exeter/30 July 2013 (Created page with "== Miniprep, NanoDrop, SureClean of RBS + Cph8 (K592018)== NanoDrop + Sureclean data: {| class="wikitable" |- ! Culture !! Quickstart protocol (ng/ul) !! Sureclean (ng/ul) |- |...")
- 11:35, 6 August 2013 (diff | hist) N Exeter/31 July 2013 (Created page with "== Liquid cultures from the 30/07/2013 == {| class="wikitable" |- ! Liquid culture !! Did it work? |- | K592018 || yes |- | B0015 || yes |- | K592022 || no |- | K864404 || no |-...")
- 11:22, 6 August 2013 (diff | hist) Exeter/29 July 2013 (→Digest)
- 11:22, 6 August 2013 (diff | hist) N Exeter/29 July 2013 (Created page with "== Liquid cultures == We made liquid cultures of: Cph8 + RBS - K592018 B0015 - Terminator K592022 - Cyan (w/RBS and promoter) K864404 - Promoter, RBS and cyan K592011 - Cy...")
- 11:47, 25 July 2013 (diff | hist) Exeter/24 July 2013
- 11:26, 25 July 2013 (diff | hist) N Exeter/24 July 2013 (Created page with " == Minipreps using the Qiagen kit == We miniprepped: * CcaR * Promoter, RBS x 3 replicates * CcaS * B0034 * Magenta * OmpR promoter * OmpR * YF1 * Lambda inverta * F...")
- 11:15, 25 July 2013 (diff | hist) File:Exeter-felicity-heath.jpg
- 11:14, 25 July 2013 (diff | hist) File:Exeter-felicity-heath.jpg
- 14:17, 24 July 2013 (diff | hist) Team:Exeter/Project (→The Biology)
- 14:33, 23 July 2013 (diff | hist) Team:Exeter/Project (→Colour system)
- 12:15, 23 July 2013 (diff | hist) Team:Exeter/Notebook (→Rachel)
- 15:37, 19 July 2013 (diff | hist) Team:Exeter/Brainstorming (→Brainstorming)
- 15:08, 19 July 2013 (diff | hist) Team:Exeter/Brainstorming (→Brainstorming)
- 11:51, 19 July 2013 (diff | hist) Team:Exeter/Brainstorming (→Brainstorming)
- 11:47, 19 July 2013 (diff | hist) Team:Exeter/Brainstorming (→Brainstorming)
- 10:51, 19 July 2013 (diff | hist) Team:Exeter/Brainstorming (→Brainstorming)
- 10:51, 19 July 2013 (diff | hist) Team:Exeter/Brainstorming (→Brainstorming)
- 10:49, 19 July 2013 (diff | hist) Team:Exeter/Brainstorming
- 10:47, 19 July 2013 (diff | hist) Team:Exeter/Project (→Introduction)
- 10:43, 19 July 2013 (diff | hist) N Team:Exeter/Website (moved Team:Exeter/Website to Team:Exeter/Brainstorming) (top)
- 10:43, 19 July 2013 (diff | hist) m Team:Exeter/Brainstorming (moved Team:Exeter/Website to Team:Exeter/Brainstorming)
- 10:42, 19 July 2013 (diff | hist) N Team:Exeter/Brainstorming (Created page with "• Allergy testing/quality assurance: Imagine you’re allergic to a certain food stuff, and you’d love to go to a new restaurant or visit a friend’s dinner party, but you c...")
- 10:38, 19 July 2013 (diff | hist) N Brainstorm (moved Brainstorm to Website over redirect) (top)
- 10:38, 19 July 2013 (diff | hist) m Website (moved Brainstorm to Website over redirect) (top)
- 10:37, 19 July 2013 (diff | hist) Website (Blanked the page)
- 10:35, 19 July 2013 (diff | hist) m Website (moved Website to Brainstorm)
- 10:33, 19 July 2013 (diff | hist) N Website (Created page with " == Brainstorm == • Allergy testing/quality assurance: Imagine you’re allergic to a certain food stuff, and you’d love to go to a new restaurant or visit a friend’s dinn...")
- 10:21, 19 July 2013 (diff | hist) Team:Exeter/Project (→The Biology)
- 10:19, 19 July 2013 (diff | hist) Exeter/18 July 2013 (→Re-streak plating)
- 10:18, 19 July 2013 (diff | hist) Exeter/18 July 2013 (→Re-streak plating)
- 10:18, 19 July 2013 (diff | hist) Exeter/18 July 2013 (→Re-streak plating)
- 10:16, 19 July 2013 (diff | hist) Exeter/18 July 2013 (→Re-streak plating)
- 09:52, 19 July 2013 (diff | hist) Exeter/18 July 2013 (→NanoDrop data from yesterday's gel extraction)
- 15:53, 18 July 2013 (diff | hist) File:Paint by coli.png (uploaded a new version of "File:Paint by coli.png") (top)
- 15:48, 18 July 2013 (diff | hist) Team:Exeter
- 15:37, 18 July 2013 (diff | hist) N File:Paint by coli.png (Logo)
- 15:15, 17 July 2013 (diff | hist) Team:Exeter/Project
- 15:14, 17 July 2013 (diff | hist) Team:Exeter/Project
- 14:59, 17 July 2013 (diff | hist) Team:Exeter/Project (→The future)
- 14:47, 17 July 2013 (diff | hist) Team:Exeter/Project (→The future)
- 14:47, 17 July 2013 (diff | hist) Team:Exeter/Project (→Blue light pathway)
- 14:40, 17 July 2013 (diff | hist) Team:Exeter/Project (→Red light pathway)
- 14:33, 17 July 2013 (diff | hist) Team:Exeter/Project (→Green light pathway)
- 14:19, 17 July 2013 (diff | hist) Team:Exeter/Project (→Blue light pathway)
- 14:14, 17 July 2013 (diff | hist) Team:Exeter/Project (→The Biology)
- 13:57, 17 July 2013 (diff | hist) Team:Exeter/Project (→The Biology)
- 13:45, 17 July 2013 (diff | hist) Team:Exeter/Project (→The Biology)
- 13:25, 17 July 2013 (diff | hist) Team:Exeter/Project (→Colour system)
- 14:54, 15 July 2013 (diff | hist) Team:Exeter/Team
- 14:53, 15 July 2013 (diff | hist) Team:Exeter/Team
- 14:51, 15 July 2013 (diff | hist) Team:Exeter/Team
- 14:46, 15 July 2013 (diff | hist) Team:Exeter/Team
- 14:45, 15 July 2013 (diff | hist) Team:Exeter/Team
- 14:43, 15 July 2013 (diff | hist) Team:Exeter/Team
- 14:42, 15 July 2013 (diff | hist) Team:Exeter/Team
- 11:04, 15 July 2013 (diff | hist) Exeter/4 July 2013 (→Afternoon)
- 10:38, 15 July 2013 (diff | hist) Exeter/4 July 2013 (→Morning)
- 10:35, 15 July 2013 (diff | hist) N Exeter/4 July 2013 (Created page with " == Morning == * Tried to make clear agar using : 5g NaCl } 15g Tryptone } Makes 1 litre 15g Agar } 5g Soytone } Tryptone, NaCl and so...")
- 10:23, 15 July 2013 (diff | hist) N Exeter/1 July 2013 (Created page with "* Took a tour of the Cat 2 lab that we are based in. * We were given lab coats and basic equipment that we will need for our project.")
- 10:16, 15 July 2013 (diff | hist) Team:Exeter/Team
- 10:15, 15 July 2013 (diff | hist) Team:Exeter/Team
- 10:15, 15 July 2013 (diff | hist) Team:Exeter/Team
- 10:47, 1 July 2013 (diff | hist) Team:Exeter/Team (→About the University of Exeter)
- 10:20, 1 July 2013 (diff | hist) Team:Exeter/Team (→Who we are)
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