User contributions
From 2013.igem.org
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- 14:01, 12 July 2013 (diff | hist) Team:Groningen/CSS
- 13:50, 12 July 2013 (diff | hist) Team:Groningen/CSS
- 13:49, 12 July 2013 (diff | hist) Team:Groningen/CSS
- 13:45, 12 July 2013 (diff | hist) Team:Groningen/CSS
- 13:45, 12 July 2013 (diff | hist) Team:Groningen/Templates/Navigationbar
- 13:44, 12 July 2013 (diff | hist) Team:Groningen/CSS
- 13:44, 12 July 2013 (diff | hist) Team:Groningen/Templates/Navigationbar
- 13:43, 12 July 2013 (diff | hist) Team:Groningen/Templates/Navigationbar
- 13:40, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 13:40, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 13:39, 12 July 2013 (diff | hist) Team:Groningen/Templates/Navigationbar
- 13:38, 12 July 2013 (diff | hist) N Team:Groningen/Templates/Navigationbar (Created page with "<ul> <li><a href="default.asp">Home</a></li> <li><a href="news.asp">News</a></li> <li><a href="contact.asp">Contact</a></li> <li><a href="about.asp">About</a></li> </ul>")
- 12:33, 12 July 2013 (diff | hist) Team:Groningen/CSS
- 12:33, 12 July 2013 (diff | hist) Team:Groningen/CSS
- 12:33, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:32, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:32, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:22, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:22, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:22, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:19, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:19, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:17, 12 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 12:16, 12 July 2013 (diff | hist) N Team:Groningen/CSS (Created page with "- →MENU bar: #main_nav { list-style: none; margin: 0; padding: 0; } #main_nav li { float: left; } #main_nav li a { text-indent: -999999px; overflow: hidden; display: block; hei...")
- 12:00, 11 July 2013 (diff | hist) Team:Groningen/Notebook
- 11:59, 11 July 2013 (diff | hist) Team:Groningen/Notebook
- 11:58, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/10 July 2013 (Created page with "The colonies of yesterday didn't grow. <br/>'''Claudio''' <br/>Made a new transformation reaction. Protocol as mentioned 08-07-2013. '''Mirjam''' <br/>Another try to grow the c...")
- 11:58, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/9 July 2013 (Created page with "'''Mirjam''' <br/>PCR made for silk without strep-tags. <br/>Gel run for 22 min 90V. <br/>Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min ...")
- 11:58, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/8 July 2013 (Created page with "'''Mirjam''' <br/> Measured the concentration of the promoter: 20,1 ng/ul. Made a restriction digestion for the promoter and the BBa_K823023 backbone. <br/>The following protoc...")
- 11:57, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/4 July 2013 (Created page with "'''Sander and Inne''' <Br/>For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the stan...")
- 11:57, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/3 July 2013 (Created page with "'''Mirjam''' <br/>Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes. '''Mirjam and Sander''' <br/>The gel examined that again dimerization ...")
- 11:56, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/2 July 2013 (Created page with """Sander"" <br/> Purified the promotor and placed it in the freezer.")
- 11:56, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/1 July 2013 (Created page with "'''Mirjam''' <br/>Made a PCR reaction for the promoter. Using the same PRC protocol as 28-06-2013. This time in the PCR mix the template DNA is the PCR product of friday and inst...")
- 11:55, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/28 June 2013 (Created page with "'''Mirjam''' <br/>Examination of all PCR products made on 27-06-2013. The silk product is run over a 0.8% agarose gel at 90V for 24 minutes. <br/>The signal sequences are run ov...")
- 11:55, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/27 June 2013 (Created page with "'''Mirjam''' <br/>Today the PCR products of the signal sequences (FliZ, EstA, MotB and LytB) are purified using gel purification (Roche gel purification kit). <br/>FliZ 9,2 ng/u...")
- 11:55, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/26 June 2013 (Created page with "'''Mirjam''' <br/>Extraction of the genomic DNA of ''B. subtilis'' strain 168. Diluting the primers (to 100 mM and 10 mM) <br/>Diluting the dNTPs to a concentration of 10 mM. ...")
- 11:54, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/25 June 2013 (Created page with "'''Mirjam, Chaline and Inne''' <br/>A couple of media are prepared: <br/>LB medium with agar 1 liter <br/>LB medium divided into 80 ml portions <br/>70% ethanol <br/>2 liter TBE ...")
- 11:54, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/20 June 2013 (Created page with "'''Mirjam, Inne and Erik''' <br/>Added 1 ul Serva/100 ml 1.5% agarose gel. <br/>Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ wat...")
- 11:09, 11 July 2013 (diff | hist) N Team:Groningen/Labwork/12 June 2013 (Created page with "== LAB CONSTRUCTS DESIGN == Today we worked at the design of constructs. This is an overview: design")
- 14:33, 10 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 14:31, 10 July 2013 (diff | hist) N File:IGEM Groingen 2013 base.css.txt (CSS file for iGEM_Groningen_2013_base) (top)
- 14:15, 10 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 14:06, 10 July 2013 (diff | hist) Team:Groningen/Labwork/18 June 2013
- 14:05, 10 July 2013 (diff | hist) N Team:Groningen/Labwork/18 June 2013 (Created page with "Mirjam, Inne and Mike Made 0.8% and 1.5% agarose serva gel solution (dissolved in TBE buffer).")
- 14:04, 10 July 2013 (diff | hist) Team:Groningen/Notebook
- 14:01, 10 July 2013 (diff | hist) Team:Groningen/Notebook
- 13:59, 10 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 10:22, 10 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 10:18, 10 July 2013 (diff | hist) Team:Groningen/TESTPAGE
- 09:59, 10 July 2013 (diff | hist) Team:Groningen/TESTPAGE
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