Team:BYU Provo/Notebook/Cholera - Enzymes/May-June

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Cholera - Enzyme Notebook May - June



Cholera Enzyme
March-April
May-June
July-August
September-October
Protocols

May 1 - May 14


We started contacting teams that have worked with Savinase and DspB. We started the initial work of purifying AmyA which was available at BYU. As well we started outlining the biofilm degradation assay using positive and negative controls.


Daily log


BYUCEFall1.JPG
May 15 - May 31


Continued our work of cloning AmyA and Savinase into E. coli. Ran initial biofilm degradation assays using urea as a positive control.


Daily log



BiohazardBYU2013.jpg
June 1 - June 14


We are still having issues with AmyA and Savinase. We are starting to fine tune our biofilm degradation assay.


Daily log



Michael pipetteFinal.jpg
June 15 - June 30


After a few more failed attempts with Savinase we are restarting the isolation from B. subtilis. AmyA is almost finished and ready for purification. The biofilm degradation assay is still being fine tuned.


Daily log



BYUCEWinter5.JPG