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Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols
From 2013.igem.org
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*98°C for 02:00 | *98°C for 02:00 | ||
Repeat the next three steps 35X | Repeat the next three steps 35X | ||
| - | + | * 98°C for 00:30 | |
| - | + | * 65°C for 00:30 | |
| - | + | * 72°C for 04:00 | |
*72°C for 8:00 | *72°C for 8:00 | ||
Hold at 4°C | Hold at 4°C | ||
Revision as of 21:42, 27 September 2013
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Cholera-Enzyme Protocols
Salt LB recipe
To 950 ml distilled H2O add
Adjust pH to 7.8
Fill to 1000 ml and autoclave
Phusion PCR (50ul reaction)
For each sample add:
Set the PCR machine to run the following cycle
Repeat the next three steps 35X
Hold at 4°C
Taq polymerase PCR (50ul reaction)
For each sample add:
95 for 2 95 for .5 50 for .5 72 for 1 72 for 1
===Any deviations from these protocols are listed in the notebook when they were used=== |
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