Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog

From 2013.igem.org

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{| width="100%"
{| width="100%"
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage July - August Notebook: July 16 - July 31 Daily Log'''</font>
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| colspan="3" | <font color="#333399" size="5" font face="Calibri">  
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: '''Small Phage July - August Notebook: July 16 - July 31 Daily Log'''</font>
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<br>
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<font color="#333399" size="3" font face="Calibri">
<font color="#333399" size="3" font face="Calibri">
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: [[Team:BYU_Provo/Small_Phage|Overview]]
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<font size = "4">
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: <u> '''Small Phage''' </u> </font>
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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<font size="4"> '''7/17/13''' </font>
<font size="4"> '''7/17/13''' </font>
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- The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for [[Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation]]
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- The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for [[Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation|7.13 Phage Propagation Experiment]]
- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.
- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.
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<br>
<br>
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<font size="4"> '''7/3/13''' </font>
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<font size="4"> '''7/19/13''' </font>
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- Performed spot test for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27 Mutagen Concentration Test - Third Protocol]] to determine phage viability. 5.3 stock and phage from [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Second Protocol]] were used as positive control.
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- Performed spot test for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.17 Mutagen Concentration Test - Fifth Protocol|7.17 Mutagen Concentration Test - Fifth Protocol]]
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- Worked on the introduction for our outreach presentation.
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- Performed titer to determine phage concentration 2 for [[Team:BYU Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13 Phage Propagation|7.13 Phage Propagation Experiment]]
<br>
<br>
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<font size="4"> '''7/7/13''' </font>
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<font size="4"> '''7/22/13''' </font>
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- Started 60mL of BL21 liquid culture overnight.
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- A lot of ousekeeping:
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: Went over parental guide, and pre-/post- questions for [[Team:BYU Provo/Outreach/Baxtor|The Adventure of Baxtor Bacteria]]
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: Discussed next steps
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: Cleanned out fridge and took pictures of plates
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: Updated website
<br>
<br>
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<font size="4"> '''7/8/13''' </font>
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<font size="4"> '''7/26/13''' </font>
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- Made 750mL of x8 top agar.
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- Designed protocols for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]] and decided to conduct an experiment to verify bacteria growth curve (specifics see [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Exp/7.27 Bacteria Growth Curve After Dilution|7.27 Bacteria Growth Curve After Dilution]])
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- We started our fourth round of mutagenesis in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.8 Mutagen Concentration Test - Forth Protocol|7.8 Mutagen Concentration Test - Fourth Protocol]]
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- Started 3mL of BL21 liquid culture overnight.
<br>
<br>
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<font size="4"> '''7/9/13''' </font>
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<font size="4"> '''7/27/13''' </font>
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- Outreach @ Orem Public Library!
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- Conducted [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Exp/7.27 Bacteria Growth Curve After Dilution|7.27 Bacteria Growth Curve After Dilution]]
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- Started approximately 20mL of E coli BL21 liquid culture overnight.
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<br>
<br>
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<font size="4"> '''7/10/13''' </font>
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<font size="4"> '''7/28/13''' </font>
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- Performed titer to determine mutated phage concentration for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.8 Mutagen Concentration Test - Forth Protocol|7.8 Mutagen Concentration Test - Fourth Protocol]]
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<br>
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<font size="4"> '''7/11/13''' </font>
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- Started 15mL of BL21 liquid culture overnight.
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- Started approximately 10mL of E coli BL21 liquid culture overnight.
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- Started phage liquid culture to test for superinfection.
<br>
<br>
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<font size="4"> '''7/12/13''' </font>
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<font size="4"> '''7/29/13''' </font>
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- Performed spot test to confirm the titer result from July 10th. Detail procedure listed in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.8 Mutagen Concentration Test - Forth Protocol|7.8 Mutagen Concentration Test - Fourth Protocol]].
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- Performed part 1 of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]].
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- Started approximately 6 mL of E coli liquid culture overnight.
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- Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)
<br>
<br>
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<font size="4"> '''7/13/13''' </font>
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<font size="4"> '''7/31/13''' </font>
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- Set up the phage liquid culture for propagation in [[Team:BYU Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13 Phage Propagation|7.13 Phage Propagation Experiment]].
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- Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]].
<br>
<br>
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<font size="4"> '''7/14/13''' </font>
 
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- Started approximately 30mL of E coli BL21 liquid culture overnight.
 
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<br>
 
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</font>
 
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{{TeamBYUProvoFooter}}
{{TeamBYUProvoFooter}}

Latest revision as of 15:35, 9 September 2013


Small Phage July - August Notebook: July 16 - July 31 Daily Log



Small Phage
March-April
May-June
July-August
September-October

7/16/13

- Started approximately 60mL of BL21 liquid culture overnight.


7/17/13

- The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for 7.13 Phage Propagation Experiment

- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.


7/19/13

- Performed spot test for 7.17 Mutagen Concentration Test - Fifth Protocol

- Performed titer to determine phage concentration 2 for 7.13 Phage Propagation Experiment


7/22/13

- A lot of ousekeeping:

Went over parental guide, and pre-/post- questions for The Adventure of Baxtor Bacteria
Discussed next steps
Cleanned out fridge and took pictures of plates
Updated website


7/26/13

- Designed protocols for 7.29 Mutagen Concentration Test - Sixth Protocol and decided to conduct an experiment to verify bacteria growth curve (specifics see 7.27 Bacteria Growth Curve After Dilution)

- Started 3mL of BL21 liquid culture overnight.


7/27/13

- Conducted 7.27 Bacteria Growth Curve After Dilution


7/28/13

- Started 15mL of BL21 liquid culture overnight.

- Started phage liquid culture to test for superinfection.


7/29/13

- Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol.

- Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)


7/31/13

- Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.