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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog
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| - | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage July - August Notebook: July 16 - July 31 Daily Log'''</font> | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> |
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| + | : '''Small Phage July - August Notebook: July 16 - July 31 Daily Log'''</font> | ||
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<font color="#333399" size="3" font face="Calibri"> | <font color="#333399" size="3" font face="Calibri"> | ||
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| + | : <u> '''Small Phage''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
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<font size="4"> '''7/17/13''' </font> | <font size="4"> '''7/17/13''' </font> | ||
| - | - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for [[Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation]] | + | - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for [[Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation|7.13 Phage Propagation Experiment]] |
- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage. | - Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage. | ||
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- Performed spot test for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.17 Mutagen Concentration Test - Fifth Protocol|7.17 Mutagen Concentration Test - Fifth Protocol]] | - Performed spot test for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.17 Mutagen Concentration Test - Fifth Protocol|7.17 Mutagen Concentration Test - Fifth Protocol]] | ||
| - | - Performed titer to determine phage concentration 2 for [[Team:BYU Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13 Phage Propagation | + | - Performed titer to determine phage concentration 2 for [[Team:BYU Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13 Phage Propagation|7.13 Phage Propagation Experiment]] |
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- A lot of ousekeeping: | - A lot of ousekeeping: | ||
| - | + | : Went over parental guide, and pre-/post- questions for [[Team:BYU Provo/Outreach/Baxtor|The Adventure of Baxtor Bacteria]] | |
| - | + | : Discussed next steps | |
| - | + | : Cleanned out fridge and took pictures of plates | |
| - | + | : Updated website | |
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| - | <font size="4"> '''7/ | + | <font size="4"> '''7/26/13''' </font> |
| - | - | + | - Designed protocols for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]] and decided to conduct an experiment to verify bacteria growth curve (specifics see [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Exp/7.27 Bacteria Growth Curve After Dilution|7.27 Bacteria Growth Curve After Dilution]]) |
| - | - | + | - Started 3mL of BL21 liquid culture overnight. |
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| - | <font size="4"> '''7/ | + | <font size="4"> '''7/27/13''' </font> |
| - | - | + | - Conducted [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Exp/7.27 Bacteria Growth Curve After Dilution|7.27 Bacteria Growth Curve After Dilution]] |
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| - | <font size="4"> '''7/ | + | <font size="4"> '''7/28/13''' </font> |
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| - | + | - Started 15mL of BL21 liquid culture overnight. | |
| - | - Started | + | - Started phage liquid culture to test for superinfection. |
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| - | <font size="4"> '''7/ | + | <font size="4"> '''7/29/13''' </font> |
| - | - Performed | + | - Performed part 1 of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. |
| - | - | + | - Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.) |
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| - | <font size="4"> '''7/ | + | <font size="4"> '''7/31/13''' </font> |
| - | - | + | - Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. |
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Latest revision as of 15:35, 9 September 2013
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7/16/13 - Started approximately 60mL of BL21 liquid culture overnight.
7/17/13 - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for 7.13 Phage Propagation Experiment - Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.
7/19/13 - Performed spot test for 7.17 Mutagen Concentration Test - Fifth Protocol - Performed titer to determine phage concentration 2 for 7.13 Phage Propagation Experiment
7/22/13 - A lot of ousekeeping:
7/26/13 - Designed protocols for 7.29 Mutagen Concentration Test - Sixth Protocol and decided to conduct an experiment to verify bacteria growth curve (specifics see 7.27 Bacteria Growth Curve After Dilution) - Started 3mL of BL21 liquid culture overnight.
7/27/13 - Conducted 7.27 Bacteria Growth Curve After Dilution
7/28/13 - Started 15mL of BL21 liquid culture overnight. - Started phage liquid culture to test for superinfection.
7/29/13 - Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol. - Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)
7/31/13 - Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.
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