Team:Duke/Parts
From 2013.igem.org
Biological Parts Submitted
From our library of gene constructs shown above, we have selected twelve parts to submit to the Registry of Standard Biological Parts. The twelve that were submitted are tabulated below, and are also labelled on the diagram above. These parts all contain a TEF1 promoter, YFP gene downstream, and a variable number of repressor binding sites (x1, x3) in the 5' UTR region. There are six difference repressor binding site sequences--three that are 16 nucleotides long, and three that are 20 nucleotides long--which can be targeted with our library of repressor constructs (TALEs and sgRNA-dCas9) shown in the diagram. These repressor constructs were not submitted. Click on the links below to see the documentation on the Registry.
Data For Our Favorite New Parts
Reporter with 1x16A Repressor Binding Sequence, BBa_K1204013 : short description
Reporter with 3x16A Repressor Binding Sequence, BBa_K1204014 ?? : short description
Reporter with 1x20A Repressor Binding Sequence, BBa_K1204016 ?? : short description
<groupparts>iGEM013 Duke</groupparts>
(Disregard the last row entry)