Team:Evry/Notebook/w10

From 2013.igem.org

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<li>Fur Binding Site 1 to 15
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<li>Fur Binding Site 1 to 15 (5 µM)
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<li>Andersen promoter J23100
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<li>Andersen promoter J23100 (5 µM)
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<li>RBS 0034
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<li>RBS 0034 (5 µM)
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Revision as of 07:41, 26 August 2013

Iron coli project

Week 10: 19th August - 25th August

2nd Construction

Primer hybridation

After sequencing analysis we observed that our Golden Gate results were not significant for the 2nd construction.
However, our sequences of natural promoter give us good results. Then we supposed that the problem of our Golden Gate come from the hybridation of our synthetic promoters.
We made new hybridations of:

  • Fur Binding Site 1 to 15 (5 µM)
  • Andersen promoter J23100 (5 µM)
  • RBS 0034 (5 µM)

Other

We prepared BL21 competent cells.
We launched Golden Gate 3 again.
Isolation and culture of 4 colonies of Fur 1, 2, 3 with sfGFP or LacI.

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