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Team:Evry/Notebook/w8
From 2013.igem.org
Week 8: 5th August - 11th August
Plasmide 3:
We started the week by doing the golden gates over again, meaning the GG 3 and GG 2 (2.1 with FurBS 1, 2.2 with FurBS2 and 2.3 with FurBS3). In fact, our previous control positive had some unwanted white spots (01/08/13), thus suggesting some contaminations during the transformation step. curretnmy waiting
Additionnally, we did the optimization of our PCR over with the temperature gradient for the annealing step. current waiting
