Team:Evry/Primers

From 2013.igem.org

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<html>
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<a href='https://2013.igem.org/Team:Evry/Amorces' title='Vers la page française'> <img src='https://static.igem.org/mediawiki/2013/b/b9/Francais.jpg'/></a>
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<div id="mainTextcontainer">
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<h1 style="text-align:center"> Primers </h1>
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<!--<a href='https://2013.igem.org/Team:Evry/Amorces' title='Vers la page française'> <img src='https://static.igem.org/mediawiki/2013/b/b9/Francais.jpg'/></a>-->
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<table width="100%" cellpadding="10" cellspacing="0" align='center' border="1">
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<h1> Primers </h1>
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<tr>   
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<th align="center">
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<b>CODE</b>
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</th>   
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<th class="colonne2" align="center" id="col2">
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<div style="width: 200px;"><b>NAME</b></div>
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</th>   
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<th align="center">
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<b>LENGTH</b>
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</th>   
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<th align="center">
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<b>ANNEALING<br/>TEMPERATURE</b>
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</th>
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<th align="center" width="200px">
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<div style="width:"200px";"<b>SEQUENCE </b></div>
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</th>
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</tr>
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<tr>  
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<table id="primer" cellpadding="10" cellspacing="0" align='center' border="1">
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<td align="center">
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  <thead>
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<p>  
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    <tr>
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P001
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      <th align="center"> <b>CODE</b> </th>  
-
</p>
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      <th class="taille1" align="center"> <b>NAME</b> </th>  
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</td>
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      <th align="center"> <b>LENGTH</b> </th>  
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<td align="center">
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      <th align="center"> <b>T<sub>a</sub></b> </th>
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<p>  
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      <th align="center" width="20px"> <b>SEQUENCE </b> </th>
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Primer Fur<br/>(Forward - GoldenBrick sequence)
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    </tr>
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</p>
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  </thead>
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</td> 
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<td align="center">
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<p>  
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41 nt
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</p>
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</td>
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<td align="center">
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<p>  
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74°C
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</p>
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</td>  
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<td>
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<p>  
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CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC
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</p>
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</td>
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</tr>
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<tr>     
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  <tbody>
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<td align="center">
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  <tr>     
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<p>  
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    <td align="center">
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P002
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      <p>  
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</p>
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        P001
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</td>  
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      </p>
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<td align="center">
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    </td>
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<p>  
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    <td align="center">
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Primer Fur<br/>(Reverse - GoldenBrick sequence)
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      <p>  
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</p>
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        Primer Fur<br/>(Forward - GoldenBrick sequence)
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</td>  
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      </p>
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<td align="center">
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    </td>
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<p>  
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    <td align="center">
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48 nt
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      <p>  
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</p>
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        41 nt
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</td>  
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      </p>
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<td align="center">
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    </td>
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<p>  
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    <td align="center">
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77°C
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      <p>  
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</p>
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        74°C
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</td>     
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      </p>
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<td>
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    </td>     
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<p>  
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    <td>
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GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG
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      <p>  
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</p>
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        CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC
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</td>
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      </p>
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</tr>
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    </td>
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  </tr>
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<tr>     
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  <tr>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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P005
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        P002
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</p>
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      </p>
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</td>     
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    </td>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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RBS B0034<br/>(Forward - GoldenBrick sequence)
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        Primer Fur<br/>(Reverse - GoldenBrick sequence)
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</p>
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      </p>
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</td>     
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    </td>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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47 nt
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        48 nt
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</p>
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      </p>
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</td>     
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    </td>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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75°C
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        77°C
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</p>
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      </p>
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</td>     
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    </td>     
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<td>
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    <td>
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<p>  
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      <p>  
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GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA
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        GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG
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</p>
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      </p>
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</td>
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    </td>
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</tr>
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  </tr>
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<tr>     
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  <tr>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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P006
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        P005
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</p>
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      </p>
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</td>     
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    </td>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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RBS B0034<br/>(Reverse - GoldenBrick sequence)
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        RBS B0034<br/>(Forward - GoldenBrick sequence)
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</p>
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      </p>
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</td>     
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    </td>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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47 nt
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        47 nt
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</p>
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      </p>
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</td>     
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    </td>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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75°C
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        75°C
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</p>
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      </p>
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</td>     
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    </td>     
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<td>
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    <td>
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<p>  
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      <p>  
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TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC
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        GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA
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</p>
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      </p>
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</td>
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    </td>
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</tr>
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  </tr>
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<tr>     
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  <tr>     
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<td align="center">
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    <td align="center">
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<p>  
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      <p>  
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P007
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        P006
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</p>
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      </p>
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</td>     
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    </td>   
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<td align="center">
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    <td align="center">
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<p>  
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      <p>
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aceB Natural Fur promoter<br/>(Forward - GoldenBrick sequence)
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        RBS B0034<br/>(Reverse - GoldenBrick sequence)
-
</p>
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      </p>
-
</td>     
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    </td>   
-
<td align="center">
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    <td align="center">
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<p>  
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      <p>
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42 nt
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        47 nt
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</p>
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      </p>
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</td>     
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    </td>   
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<td align="center">
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    <td align="center">
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<p>  
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      <p>
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76°C
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        75°C
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</p>
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      </p>
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</td>     
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    </td>   
-
<td>
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    <td>
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<p>  
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      <p>
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GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC
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        TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC
-
</p>
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      </p>
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</td>
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    </td>
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</tr>
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  </tr>
 +
 
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P007
 +
      </p>
 +
    </td>     
 +
    <td align="center">
 +
      <p>  
 +
        aceB Natural Fur promoter<br/>(Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>     
 +
    <td align="center">
 +
      <p>  
 +
        42 nt
 +
      </p>
 +
    </td>     
 +
    <td align="center">
 +
      <p>  
 +
        76°C
 +
      </p>
 +
    </td>     
 +
    <td>
 +
      <p>  
 +
        GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC
 +
      </p>
 +
    </td>
 +
  </tr>
   <tr>     
   <tr>     
Line 384: Line 380:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCT<br/>AGCGTGATGAGACCG
+
         GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCGTGATGAGACCG
       </p>
       </p>
     </td>
     </td>
Line 412: Line 408:
     <td>
     <td>
       <p>  
       <p>  
-
         CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAG<br/>GCTGAGACCGAATTC
+
         CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAGGCTGAGACCGAATTC
       </p>
       </p>
     </td>
     </td>
Line 1,560: Line 1,556:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCT<br/>AGCGTGATGAGACCG
+
         GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCGTGATGAGACCG
       </p>
       </p>
     </td>
     </td>
Line 1,588: Line 1,584:
     <td>
     <td>
       <p>  
       <p>  
-
         CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAG<br/>GCTGAGACCGAATTC
+
         CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAGGCTGAGACCGAATTC
       </p>
       </p>
     </td>
     </td>
Line 1,616: Line 1,612:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCT<br/>AGCGTGATGAGACCG
+
         GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCTAGCGTGATGAGACCG
       </p>
       </p>
     </td>
     </td>
Line 1,644: Line 1,640:
     <td>
     <td>
       <p>  
       <p>  
-
         CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAG<br/>GCTGAGACCGAATTC
+
         CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC
       </p>
       </p>
     </td>
     </td>
Line 1,672: Line 1,668:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCT<br/>AGCGTGATGAGACCG
+
         GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCGTGATGAGACCG
       </p>
       </p>
     </td>
     </td>
Line 1,700: Line 1,696:
     <td>
     <td>
       <p>  
       <p>  
-
         CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAG<br/>GCTGAGACCGAATTC
+
         CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC
       </p>
       </p>
     </td>
     </td>
Line 1,784: Line 1,780:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATG<br/>GATTTCAGCGGTAAAAATG
+
         GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATGGATTTCAGCGGTAAAAATG
       </p>
       </p>
     </td>
     </td>
Line 1,840: Line 1,836:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGG<br/>CTATTCCAAAATTACAGGC
+
         GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGGCTATTCCAAAATTACAGGC
       </p>
       </p>
     </td>
     </td>
Line 1,896: Line 1,892:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGG<br/>ATACGTCACTGGC
+
         GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGGATACGTCACTGGC
       </p>
       </p>
     </td>
     </td>
Line 1,952: Line 1,948:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCG<br/>ATATGAAAACTACGC
+
         GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCGATATGAAAACTACGC
       </p>
       </p>
     </td>
     </td>
Line 2,008: Line 2,004:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATG<br/>AGCATTCCATTCACCC
+
         GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATGAGCATTCCATTCACCC
       </p>
       </p>
     </td>
     </td>
Line 2,064: Line 2,060:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCA<br/>GCATTTACCTTTG
+
         GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCAGCATTTACCTTTG
       </p>
       </p>
     </td>
     </td>
Line 2,120: Line 2,116:
     <td>
     <td>
       <p>  
       <p>  
-
         CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAA<br/>AGGCGAAGAG
+
         CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAAAGGCGAAGAG
       </p>
       </p>
     </td>
     </td>
Line 2,176: Line 2,172:
     <td>
     <td>
       <p>  
       <p>  
-
         GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAG<br/>CGGATAACAAGATACTGAGCACAGAGTTCAGAGACCAC
+
         GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACAGAGTTCAGAGACCAC
       </p>
       </p>
     </td>
     </td>
Line 2,204: Line 2,200:
     <td>
     <td>
       <p>  
       <p>  
-
         GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGT<br/>TATCCGCTCACAATTCTCTAGAGGCTGAGACCGAATTC
+
         GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGTTATCCGCTCACAATTCTCTAGAGGCTGAGACCGAATTC
       </p>
       </p>
     </td>
     </td>
Line 2,320: Line 2,316:
     </td>
     </td>
   </tr>
   </tr>
 +
  </tbody>
</table>
</table>
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{{:Team:Evry/foot}}

Latest revision as of 16:32, 1 October 2013

Iron coli project

Primers

CODE NAME LENGTH Ta SEQUENCE

P001

Primer Fur
(Forward - GoldenBrick sequence)

41 nt

74°C

CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC

P002

Primer Fur
(Reverse - GoldenBrick sequence)

48 nt

77°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG

P005

RBS B0034
(Forward - GoldenBrick sequence)

47 nt

75°C

GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA

P006

RBS B0034
(Reverse - GoldenBrick sequence)

47 nt

75°C

TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC

P007

aceB Natural Fur promoter
(Forward - GoldenBrick sequence)

42 nt

76°C

GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC

P008

ace B Natural Fur promoter
(Reverse - GoldenBrick sequence)

32 nt

78°C

TGCAGGGTCTCACTAGTACGTGCAGCTCCTCG

P009

Ent C Natural Fur promoter
(Forward - GoldenBrick sequence)

49 nt

73°C

GAATTCGGTCTCAGCCTCTAGAGAAATCAGCTTCCTGTTATTAATAAGG

P010

Ent C Natural Fur promoter
(Reverse - GoldenBrick sequence)

32 nt

64°C

ACTAGTAATCATCCTCCACAAAATGATAAAGG

P011

Fec A Natural Fur promoter
(Forward - GoldenBrick sequence)

50 nt

73°C

GAATTCGGTCTCAGCCTCTAGAGGGAAAATAATTCTTATTTCGATTGTCC

P012

Fec A Natural Fur promoter
(Reverse - GoldenBrick sequence)

41 nt

74°C

CTGCAGGGTCTCACTAGTAACCTTCCCCATCATCATTTTTG

P013

Fec C Natural Fur promoter
(Forward - GoldenBrick sequence)

42 nt

77°C

GAATTCGGTCTCAGCCTCTAGAGGAACAGCAATTCAACCTGC

P014

Fec C Natural Fur promoter
(Reverse - GoldenBrick sequence)

36 nt

75°C

CTGCAGGGTCTCACTAGTATTCACAACGGTAAGCGG

P015

Andersen's promoter J23100
(Forward - GoldenBrick sequence)

70 nt

84°C

GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCGTGATGAGACCG

P016

Andersen's promoter J23100
(Reverse - GoldenBrick sequence)

70 nt

84°C

CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAGGCTGAGACCGAATTC

P017

Fur Binding Site N°1
(Foward - GoldenBrick sequence)

45 nt

72°C

CGGTCTCTGTGAATTATTGATAACTATTTGGATGCTAGAGACCAC

P018

Fur Binding Site N°1
(Reverse - GoldenBrick sequence)

45 nt

72°C

GTGGTCTCTAGCATCCAAATAGTTATCAATAATTCACAGAGACCG

P019

Fur Binding Site N°2
(Foward - GoldenBrick sequence)

47 nt

75°C

CGGTCTCTGTGAGATAACTATTTGCATTTGCAGATGCTAGAGACCAC

P020

Fur Binding Site N°2
(Reverse - GoldenBrick sequence)

47 nt

75°C

GTGGTCTCTAGCATCTGCAAATGCAAATAGTTATCTCACAGAGACCG

P021

Fep A Natural Fur promoter
(Foward - GoldenBrick sequence)

41 nt

76°C

GAATTCGGTCTCAGCCTCTAGAGTTCGTCATTCAGACGCTG

P022

Fep A Natural Fur promoter
(Reverse - GoldenBrick sequence)

42 nt

73°C

CTGCAGGGTCTCACTAGTATGTTTTATTCCTGCATTTTTGCC

P023

Fes Natural Fur promoter
(Foward - GoldenBrick sequence)

40 nt

77°C

GAATTCGGTCTCAGCCTCTAGAGGCCACGAATTGCAACTG

P024

Fes Natural Fur promoter
(Reverse - GoldenBrick sequence)

37 nt

75°C

CTGCAGGGTCTCACTAGTATTCGTCATTCAGACGCTG

P025

Sdh C Natural Fur promoter
(Foward - GoldenBrick sequence)

45 nt

75°C

GAATTCGGTCTCAGCCTCTAGAGATCACTGTTCAGGATAAAACCC

P026

Sdh C Natural Fur promoter
(Reverse - GoldenBrick sequence)

36 nt

76°C

CTGCAGGGTCTCACTAGTAGAATAACGCCCACATGC

P027

ybi L Natural Fur promoter
(Forward - GoldenBrick sequence)

50 nt

76°C

GAATTCGGTCTCAGCCTCTAGAGCACCTAATTTGCCCATGATGATATCTC

P028

ybi L Natural Fur promoter
(Reverse - GoldenBrick sequence)

50 nt

72°C

CTGCAGGGTCTCACTAGTAGTGATGGCTTCTGATAAAGATAAATTTATAG

P029

ync E Natural Fur promoter
(Forward - GoldenBrick sequence)

40 nt

78°C

GAATTCGGTCTCAGCCTCTAGAGACGGGGGAAAGGACAAG

P030

ync E Natural Fur promoter
(Reverse - GoldenBrick sequence)

39 nt

75°C

CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC

P031

Fur Binding Site N°3
(Forward - GoldenBrick sequence)

47 nt

75°C

CGGTCTCTGTGACAAATGCAAATAGTTATCCAGATGCTAGAGACCAC

P032

Fur Binding Site N°3
(Reverse - GoldenBrick sequence)

47 nt

75°C

GTGGTCTCTAGCATCTGGATAACTATTTGCATTTGTCACAGAGACCG

P033

Fur Binding Site N°4
(Forward - GoldenBrick sequence)

47 nt

72°C

CGGTCTCTGTGACAAATAGTTATCAATAATCAGATGCTAGAGACCAC

P034

Fur Binding Site N°4
(Reverse - GoldenBrick sequence)

47 nt

72°C

GTGGTCTCTAGCATCTGATTATTGATAACTATTTGTCACAGAGACCG

P035

Fur Binding Site N°5
(Forward - GoldenBrick sequence)

47 nt

70°C

CGGTCTCTGTGAGTAAATTAATATTATTTACAGATGCTAGAGACCAC

P036

Fur Binding Site N°5
(Reverse - GoldenBrick sequence)

47 nt

70°C

GTGGTCTCTAGCATCTGTAAATAATATTAATTTACTCACAGAGACCG

P037

Fur Binding Site N°6
(Forward - GoldenBrick sequence)

47 nt

74°C

CGGTCTCTGTGAAATAATGCTTCTCATTTTCAGATGCTAGAGACCAC

P038

Fur Binding Site N°6
(Reverse - GoldenBrick sequence)

47 nt

74°C

GTGGTCTCTAGCATCTGAAAATGAGAAGCATTATTTCACAGAGACCG

P039

Fur Binding Site N°7
(Forward - GoldenBrick sequence)

47 nt

71°C

CGGTCTCTGTGAATAAATGATAATCATTATCAGATGCTAGAGACCAC

P040

Fur Binding Site N°7
(Reverse - GoldenBrick sequence)

47 nt

71°C

GTGGTCTCTAGCATCTGATAATGATTATCATTTATTCACAGAGACCG

P041

Fur Binding Site N°8
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGAGAAAATAATTCTTATTTCCAGATGCTAGAGACCAC

P042

Fur Binding Site N°8
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGGAAATAAGAATTATTTTCTCACAGAGACCG

P043

Fur Binding Site N°9
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGAAGCACTTATTATTATTTTCAGATGCTAGAGACCAC

P044

Fur Binding Site N°9
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGAAAATAATAATAAGTGCTTCACAGAGACCG

P045

Fur Binding Site N°10
(Forward - GoldenBrick sequence)

47 nt

75°C

CGGTCTCTGTGAGATAATTGTTATCGTTTGCAGATGCTAGAGACCAC

P046

Fur Binding Site N°10
(Reverse - GoldenBrick sequence)

47 nt

75°C

GTGGTCTCTAGCATCTGCAAACGATAACAATTATCTCACAGAGACCG

P047

Fur Binding Site N°11
(Forward - GoldenBrick sequence)

47 nt

74°C

CGGTCTCTGTGAGATAATGCTTATCAAAATCAGATGCTAGAGACCAC

P048

Fur Binding Site N°11
(Reverse - GoldenBrick sequence)

47 nt

74°C

GTGGTCTCTAGCATCTGATTTTGATAAGCATTATCTCACAGAGACCG

P049

Fur Binding Site N°12
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGACAAAATTATTATCACTTTCAGATGCTAGAGACCAC

P050

Fur Binding Site N°12
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGAAAGTGATAATAATTTTGTCACAGAGACCG

P051

Fur Binding Site N°13
(Forward - GoldenBrick sequence)

47 nt

75°C

CGGTCTCTGTGAAATAATGATTACCATTCCCAGATGCTAGAGACCAC

P052

Fur Binding Site N°13
(Reverse - GoldenBrick sequence)

47 nt

75°C

GTGGTCTCTAGCATCTGGGAATGGTAATCATTATTTCACAGAGACCG

P053

Fur Binding Site N°14
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGAGAAATTGTTTTTGATTTTCAGATGCTAGAGACCAC

P054

Fur Binding Site N°14
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGAAAATCAAAAACAATTTCTCACAGAGACCG

P055

Fur Binding Site N°15
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGAGTTAATTGTAATGATTTTCAGATGCTAGAGACCAC

P056

Fur Binding Site N°15
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGAAAATCATTACAATTAACTCACAGAGACCG

P057

Andersen's promoter J23101
(Forward - GoldenBrick sequence)

70 nt

81°C

GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCGTGATGAGACCG

P058

Andersen's promoter J23101
(Reverse - GoldenBrick sequence)

70 nt

81°C

CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAGGCTGAGACCGAATTC

P059

Andersen's promoter J23102
(Forward - GoldenBrick sequence)

70 nt

83°C

GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCTAGCGTGATGAGACCG

P060

Andersen's promoter J23102
(Reverse - GoldenBrick sequence)

70 nt

83°C

CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC

P061

Andersen's promoter J23104
(Forward - GoldenBrick sequence)

70 nt

82°C

GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCGTGATGAGACCG

P062

Andersen's promoter J23104
(Reverse - GoldenBrick sequence)

70 nt

82°C

CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC

P063

VR primer iGEM standard

20 nt

63°C

ATTACCGCCTTTGAGTGAGC

P064

VF2 primer iGEM standard

20 nt

64°C

TGCCACCTGACGTCTAAGAA

P065

Ent A Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

74 nt

81°C

GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATGGATTTCAGCGGTAAAAATG

P066

Ent A Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

45 nt

81°C

GGACTGCAGACTAGTGGTCTCTTGGATCTTATGCCCCCAGCGTTG

P067

Ent B Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

74 nt

78°C

GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGGCTATTCCAAAATTACAGGC

P068

Ent B Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

45 nt

79°C

GGACTGCAGACTAGTGGTCTCTTGGATCTTATTTCACCTCGCGGG

P069

Ent C Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

68 nt

80°C

GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGGATACGTCACTGGC

P070

Ent C Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

53 nt

76°C

GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATGCAATCCAAAAACGTTCAAC

P071

Ent D Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

70 nt

83°C

GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCGATATGAAAACTACGC

P072

Ent D Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

47 nt

79°C

GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATCGTGTTGGCACAGC

P073

Ent E Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

71 nt

80°C

GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATGAGCATTCCATTCACCC

P074

Ent E Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

43 nt

81°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTCAGGCTGATGCGCG

P075

Ent F Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

68 nt

75°C

GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCAGCATTTACCTTTG

P076

Ent F Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

47 nt

78°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTACCTGTTTAGCGTTGCG

P077

RBS sfGFP - Plasmid 3
(Forward - GoldenBrick sequence)

65 nt

76°C

CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAAAGGCGAAGAG

P078

RBS sfGFP - Plasmid 3
(Reverse - GoldenBrick sequence)

58 nt

75°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTACAGTTCATCCATACCATG

P079

Promoteur Lac + Lac Operator
(Forward - GoldenBrick sequence)

93 nt

82°C

GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACAGAGTTCAGAGACCAC

P080

Promoteur Lac + Lac Operator
(Reverse - GoldenBrick sequence)

93 nt

82°C

GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGTTATCCGCTCACAATTCTCTAGAGGCTGAGACCGAATTC

P081

RBS positive control - Plasmid 2
(Forward - GoldenBrick sequence)

46 nt

77°C

CGGTCTCTGTGATGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA

P082

RBS positive control - Plasmid 2
(Reverse - GoldenBrick sequence)

46 nt

77°C

TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCATCACAGAGACCG

P083

Lac I - Plasmid 1/2
(Forward - GoldenBrick sequence)

40 nt

72°C

GAATTCGGTCTCTCTAGATGGTGAATGTGAAACCAGTAAC

P084

Lac I - Plasmid 1/2
(Reverse - GoldenBrick sequence)

56 nt

76°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCTACTAAAGCGTAGTTTTCG

Retrieved from "http://2013.igem.org/Team:Evry/Primers"