"
Team:Evry/Sensor
From 2013.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
<h1>Sensor</h1> | <h1>Sensor</h1> | ||
| + | |||
| + | <p> | ||
| + | We constructed iron sensors by combining 3 genetic parts: an ''E. coli'' promoter with a putative Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below. | ||
| + | </p> | ||
<div align='center'><img src="https://static.igem.org/mediawiki/2013/7/7b/P1-GFP.png" width="75%"/></div> | <div align='center'><img src="https://static.igem.org/mediawiki/2013/7/7b/P1-GFP.png" width="75%"/></div> | ||
| Line 20: | Line 24: | ||
</th> | </th> | ||
<th align="center"> | <th align="center"> | ||
| - | <b> | + | <b>DESCRIPTION</b> |
</th> | </th> | ||
</tr> | </tr> | ||
| Line 27: | Line 31: | ||
<td align="center"> | <td align="center"> | ||
<p> | <p> | ||
| - | + | E. coli promoter with Fur binding site | |
</p> | </p> | ||
</td> | </td> | ||
| Line 35: | Line 39: | ||
<td align="center"> | <td align="center"> | ||
<p> | <p> | ||
| - | + | iron-Fur complex binds promoter to repress expression | |
</p> | </p> | ||
</td> | </td> | ||
| Line 51: | Line 55: | ||
<td align="center"> | <td align="center"> | ||
<p> | <p> | ||
| - | + | Fluorescent reporter gene | |
</p> | </p> | ||
</td> | </td> | ||
| Line 67: | Line 71: | ||
<td align="center"> | <td align="center"> | ||
<p> | <p> | ||
| - | + | terminator to stop transcription | |
</p> | </p> | ||
</td> | </td> | ||
| Line 83: | Line 87: | ||
<td align="center"> | <td align="center"> | ||
<p> | <p> | ||
| - | + | Biobrick-compatible plasmid backbone | |
</p> | </p> | ||
</td> | </td> | ||
Revision as of 09:33, 4 October 2013
Sensor
We constructed iron sensors by combining 3 genetic parts: an ''E. coli'' promoter with a putative Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below.
| NAME | FIGURE | DESCRIPTION |
|---|---|---|
|
E. coli promoter with Fur binding site |
|
iron-Fur complex binds promoter to repress expression |
|
sfGFP |
|
Fluorescent reporter gene |
|
Terminator |
|
terminator to stop transcription |
|
Plasmid |
|
Biobrick-compatible plasmid backbone |
Site developped by Gabriel Guillocheau
