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Team:Evry/Sensor
From 2013.igem.org
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| - | We constructed iron sensors by combining 3 genetic parts: an | + | We constructed iron sensors by combining 3 genetic parts: an E. coli promoter with a Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below. |
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Revision as of 09:34, 4 October 2013
Sensor
We constructed iron sensors by combining 3 genetic parts: an E. coli promoter with a Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below.
| NAME | FIGURE | DESCRIPTION |
|---|---|---|
|
E. coli promoter with Fur binding site |
|
iron-Fur complex binds promoter to repress expression |
|
sfGFP |
|
Fluorescent reporter gene |
|
Terminator |
|
terminator to stop transcription |
|
Plasmid |
|
Biobrick-compatible plasmid backbone |
Site developped by Gabriel Guillocheau
