Team:Evry/Sensor

From 2013.igem.org

Revision as of 09:57, 4 October 2013 by Tolonen (Talk | contribs)

Iron coli project

Sensor

We constructed iron sensors by combining 3 genetic parts: an E. coli promoter with a Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below.



Fig Diagram of a genetic iron sensor. Iron bind the Ferric Uptake Regulator (Fur) to form a complex that binds the Fur box in the promoter, here shown as the aceB promoter. Once the iron-Fur complex is bound, it represses transcription of the target gene GFP.



Fig Construction of an iron-responsive genetic element by fusing a Fur-regulated promoter with a reporter gene. Promoter-reporter fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.

NAME FIGURE DESCRIPTION

E. coli promoter with Fur binding site

iron-Fur complex binds promoter to repress expression

sfGFP

Fluorescent reporter gene

Terminator

terminator to stop transcription

Plasmid

Biobrick-compatible plasmid backbone