Team:Freiburg

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   <img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
   <img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
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   <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div>
   <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div>
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     regulation in mammalian cells.  Therefore, we engineered the recently understood and highly promising   
     regulation in mammalian cells.  Therefore, we engineered the recently understood and highly promising   
     CRISPR/Cas9 system. <br>
     CRISPR/Cas9 system. <br>
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       The regulation is based on the RNA-guided Cas9 protein, which allows targeting of specific DNA sequences.  
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       The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences.  
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       Our toolkit comprises not only a standardized Cas9 protein,  but also different effector domains for  
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       Our toolkit comprises not only a standardized dCas9 protein,  but also different effector domains for  
       efficient gene activation or repression.  
       efficient gene activation or repression.  
</div>  
</div>  
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<ul style="margin-left:160px">
<ul style="margin-left:160px">
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<li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna#multiple_targeting">
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<li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna">
   <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png">
   <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png">
<span> Multiple targeting </span>
<span> Multiple targeting </span>
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       <div id="text_gross" align="left"><a style="position:absolute; top:678px; left:60px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Highlights"> Highlights </div> </a>
       <div id="text_gross" align="left"><a style="position:absolute; top:678px; left:60px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Highlights"> Highlights </div> </a>
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   <p style="position:absolute; top:720px; left:60px" > All about our <br> project on one <br> page! </p>
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   <p style="position:absolute; top:720px; left:60px" > All about our <br> project in one <br> slide show! </p>
  <img src="https://static.igem.org/mediawiki/2013/0/0e/Freiburg_2013_main2_Highlights5.png" height="190" style="position:absolute; top:614px; left:105px; z-index:1">  
  <img src="https://static.igem.org/mediawiki/2013/0/0e/Freiburg_2013_main2_Highlights5.png" height="190" style="position:absolute; top:614px; left:105px; z-index:1">  
   </div>  
   </div>  
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      <div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:910px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Team2"> Team </a></div>
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<div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:910px; z-index:2" href="https://2013.igem.org/Team:Freiburg/News"> News </a></div>
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  <img src="https://static.igem.org/mediawiki/2013/9/91/Freiburg_2013_main2_teamkachel_TEAM2.png" height="140" style="position:absolute; top:685px; left:900px; z-index:1">  
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<p style="padding-top:10px;">
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Have a look at our achievements in Lyon & Boston!
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</p>
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<img src="https://static.igem.org/mediawiki/2013/5/56/Best-foundational-advance-2013-freiburg.png" height="130" style="position:absolute; top:600px; left:980px; z-index:1">  
   </div>  
   </div>  
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</div>
   <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
   <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
   </td>
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Latest revision as of 23:52, 9 April 2014


Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising CRISPR/Cas9 system.
The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences. Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for efficient gene activation or repression.

All about our
project in one
slide show!

Use our Toolkit
to regulate your
genes!

Have a look at our achievements in Lyon & Boston!