Team:Freiburg/Highlights
From 2013.igem.org
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<li>...construct a catalytically inactive version of <b>Cas9</b> and thus generate a new <b>DNA binding protein</b>.</li> | <li>...construct a catalytically inactive version of <b>Cas9</b> and thus generate a new <b>DNA binding protein</b>.</li> | ||
- | <li>...combine this modified dCas9 with <b> | + | <li>...combine this modified dCas9 with different transcriptional <b>effectors</b>.</li> |
<li>...<b>express</b> this system in various <b>mammalian</b> cell lines.</li> | <li>...<b>express</b> this system in various <b>mammalian</b> cell lines.</li> | ||
- | <li>...<b>control | + | <li>...<b>control</b> mammalian gene expression via our modified CRISPR/Cas system.</li> |
<li>...regulate gene expression on <b>light stimulus</b>.</li> | <li>...regulate gene expression on <b>light stimulus</b>.</li> | ||
- | <li>...make our dCas9 <b>accessible to the whole iGEM community | + | <li>...make our dCas9 <b>accessible</b> to the whole iGEM community by mutating illegal iGEM restriction sites</b>.</li> |
<br> | <br> | ||
- | In summary we build up a <b> | + | In summary we build up a universal applicable <b>toolkit</b> for gene regulation. |
</ul> | </ul> | ||
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6 opportunities to customize your experiments | 6 opportunities to customize your experiments | ||
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- | <img id="main_images" src="https://static.igem.org/mediawiki/2013/0/08/Symbols_farbenfroh_freiburg_13.png" style="height: | + | <img id="main_images" src="https://static.igem.org/mediawiki/2013/0/08/Symbols_farbenfroh_freiburg_13.png" style="height:600px; margin-left:-10px; margin-top:30px;"> |
</div> | </div> |
Revision as of 00:17, 3 October 2013
HIGHLIGHTS
In the last months we were able to...
In summary we build up a universal applicable toolkit for gene regulation. |
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