Team:Freiburg/Highlights
From 2013.igem.org
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- | One of the biggest advantages of the CRISPR/Cas9 system is that only one protein is required for targeting several DNA sequences. For a new DNA target there has to be just another guiding RNA. We designed an RNA plasmid, "<a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna">RNAimer</a>", containing the sequence for this RNA. For multiple targeting different RNAimer inserts can be easily combined using the iGEM BioBrick | + | One of the biggest advantages of the CRISPR/Cas9 system is that only one protein is required for targeting several DNA sequences. For a new DNA target there has to be just another guiding RNA. We designed an RNA plasmid, "<a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna">RNAimer</a>", containing the sequence for this RNA. For multiple targeting different RNAimer inserts can be easily combined using the iGEM BioBrick assembly method. And as the figures show, if one gene is targeted at two loci at a time the activation efficiency shows an additive effect! <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna#multiple_targeting">Read more!</a> |
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Revision as of 18:53, 4 October 2013
HIGHLIGHTS