Team:Freiburg/Highlights
From 2013.igem.org
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6 opportunities with our uniCAS toolkit | 6 opportunities with our uniCAS toolkit | ||
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- | <p>We provide <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/effector">3 different effectors</a>, 2 methods & <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/induction#light"> | + | <p>We provide <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/effector">3 different effectors</a>, 2 methods & 1 effector controller! Using our toolkit it's possible to efficiently activate or repress genes in mammalian cells. We also provide devices for <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/induction#light">effector controling by light</a>. Use our custom-tailored <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/toolkit">Manual Tool</a> to generate instructions for your own gene regulation experiment. With our toolkit it's possible to <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna">target not only one</a>, but <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna#multiple_targeting">multiple genes</a> of interest! We also established <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/method">uniBAss</a> - our universal Binding Assay for assessing the binding capacity of our fusion proteins. Best of all: It's open source and in iGEM standard!</p> |
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dCas9 - The Heart of our toolkit | dCas9 - The Heart of our toolkit | ||
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- | <p>We started by <a id="link" href="https://2013.igem.org/Team:Freiburg/ | + | <p>We started by <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/1#introduction">mutating</a> the DNA cleavage site in the Cas9 protein and therefore generated a DNA binding protein. The binding mechanism is relying on a protein-RNA-DNA interaction. By changing the RNA sequence we are now able to determine the DNA binding locus and can direct the protein to any requested DNA target.</p> |
<p>This is the <b>heart of our toolkit</b>. A protein that allows multiple and sequence specified DNA binding. By fusing effector domains to dCas9 we created fusion proteins with completely new function. Read more in the next slides.</p> | <p>This is the <b>heart of our toolkit</b>. A protein that allows multiple and sequence specified DNA binding. By fusing effector domains to dCas9 we created fusion proteins with completely new function. Read more in the next slides.</p> | ||
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- | <img src="https://static.igem.org/mediawiki/2013/e/e5/Freiburg-2013-heart5.png" style="width: | + | <img src="https://static.igem.org/mediawiki/2013/e/e5/Freiburg-2013-heart5.png" style="width:500px;"> |
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- | Specific chromatin modification was achieved by fusing the histone methyltransferase G9a to dCas9 and therefore creating an epigenetic | + | Specific chromatin modification was achieved by fusing the histone methyltransferase G9a to dCas9 and therefore creating an epigenetic biobrick. G9a primarily methylates Histone H3. Different endogenous VEGF loci were targeted in mammalian cells which resulted in an up to 50 % repression (target 3 equals VEGF-8 and target 4 equals VEGF-573). <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/effector#epigenetics"> Read more!</a> |
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Revision as of 20:02, 4 October 2013
HIGHLIGHTS