Team:Freiburg/Highlights
From 2013.igem.org
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- | Specific chromatin modification was achieved by fusing the histone methyltransferase G9a to dCas9 thereby contributing an epigenetic BioBrick. G9a primarily methylates Histone H3. Different endogenous <i>vegf</i> loci were targeted in mammalian cells. This resulted in an up to 50 % repression in which target 3 corresponds to a region in the <i>vegf</i> loci at position -8 bp from the transcription start site (TSS) and target 4 equals <i>vegf</i> -573 from ths TSS. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/effector#epigenetics"> Read more!</a> | + | Specific chromatin modification was achieved by fusing the histone methyltransferase G9a to dCas9 and thereby contributing an epigenetic BioBrick. G9a primarily methylates Histone H3. Different endogenous <i>vegf</i> loci were targeted in mammalian cells. This resulted in an up to 50 % repression in which target 3 corresponds to a region in the <i>vegf</i> loci at position -8 bp from the transcription start site (TSS) and target 4 equals <i>vegf</i> -573 from ths TSS. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/effector#epigenetics"> Read more!</a> |
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Revision as of 00:57, 5 October 2013
HIGHLIGHTS