Team:Freiburg/Highlights
From 2013.igem.org
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<p id="headline"> <img src="https://static.igem.org/mediawiki/2013/e/ef/UniBAss_freiburg_13.png" style="height:65px; width:65px; margin-top:-34px; margin-left:-4px; "> | <p id="headline"> <img src="https://static.igem.org/mediawiki/2013/e/ef/UniBAss_freiburg_13.png" style="height:65px; width:65px; margin-top:-34px; margin-left:-4px; "> | ||
- | + | uniCAS Binding Assay - uniBAss | |
</p> | </p> | ||
<p>We developed a novel and innovative ELISA-based method for quantifying the binding efficiencies of our dCas9 proteins: The uniCAS binding assay uniBAss. Biotinylated oligos are coated on 96-well plates via the interaction with strepatvidin. It could be shown that it is a powerful tool for characterizing the modified dCas9 fusion proteins by assessing its DNA binding capacity with possible improvements for high-throughput screenings. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/method">Read more!</a> | <p>We developed a novel and innovative ELISA-based method for quantifying the binding efficiencies of our dCas9 proteins: The uniCAS binding assay uniBAss. Biotinylated oligos are coated on 96-well plates via the interaction with strepatvidin. It could be shown that it is a powerful tool for characterizing the modified dCas9 fusion proteins by assessing its DNA binding capacity with possible improvements for high-throughput screenings. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/method">Read more!</a> |
Revision as of 01:14, 5 October 2013
HIGHLIGHTS