Team:Freiburg/Highlights
From 2013.igem.org
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uniCAS Binding Assay - uniBAss | uniCAS Binding Assay - uniBAss | ||
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- | <p>We developed a novel and innovative ELISA-based method for quantifying the binding efficiencies of our dCas9 proteins: The uniCAS Binding Assay uniBAss. Biotinylated oligos are coated on 96-well plates via the interaction with | + | <p>We developed a novel and innovative ELISA-based method for quantifying the binding efficiencies of our dCas9 proteins: The uniCAS Binding Assay uniBAss. Biotinylated oligos are coated on 96-well plates via the interaction with streptavidin. It could be shown that it is a powerful tool for characterizing the modified dCas9 fusion proteins by assessing its DNA binding capacity with possible improvements for high-throughput screenings. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/method">Read more!</a> |
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Revision as of 01:16, 5 October 2013
HIGHLIGHTS