Team:Freiburg/Highlights
From 2013.igem.org
(Difference between revisions)
Line 360: | Line 360: | ||
</p> | </p> | ||
<p> | <p> | ||
- | The transcriptional repressor domain Krüppel associated box (KRAB) was fused to dCas9. Thus, a transcriptional repressor with the flexibility to target any DNA sequence of interest was engineered. The device was tested in mammalian cells to target endogenous VEGF loci. An up to 50% repression was achieved | + | The transcriptional repressor domain Krüppel associated box (KRAB) was fused to dCas9. Thus, a transcriptional repressor with the flexibility to target any DNA sequence of interest was engineered. The device was tested in mammalian cells to target endogenous VEGF loci. An up to 50% repression was achieved when targeting different DNA sites. The numbers characterizing the crRNAs represent the distance from the transcription start site. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/effector#repression">Read more!</a> |
</p> | </p> | ||
Line 374: | Line 374: | ||
<table id="Fig8" class="imgtxt"> | <table id="Fig8" class="imgtxt"> | ||
<tbody><tr> | <tbody><tr> | ||
- | <td> <a href="https://2013.igem.org/Team:Freiburg/Project/effector#repression"> <img src="https://static.igem.org/mediawiki/2013/ | + | <td> <a href="https://2013.igem.org/Team:Freiburg/Project/effector#repression"> <img src="https://static.igem.org/mediawiki/2013/5/5b/KRAB-Freiburg-2013.png"></a> </td> |
</tr> | </tr> | ||
Revision as of 21:16, 16 October 2013
HIGHLIGHTS