Team:Freiburg/Highlights
From 2013.igem.org
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+ | <!-- Light --> | ||
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+ | <p id="headline" > <a href="https://2013.igem.org/Team:Freiburg/Project/induction#light"> <img src="https://static.igem.org/mediawiki/2013/9/9a/Freiburg_2013_main2_Licht.png" style="height:65px; width:65px; margin-top:-34px; margin-left:-4px; "> </a> | ||
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+ | We aimed to control our system with light thereby allowing for gene regulation with high spatiotemporal resolution. We engineered a system for induction by red, UVB and blue light. The blue light system is based on the light-triggered interaction of CRY2 and CIB1. CRY2 was fused to dCas9 which can upon light-stimulus recruit the CIB1-VP16 fusion protein to any DNA sequence of interest. A 5-fold upregulation of SEAP reporter was achieved (*, p<0.05). dCas9-CRY2 was targeted to four DNA regions upstream of the promoter. The numbers characterizing the crRNAs represent the distance from the translation start site. | ||
+ | <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/induction#light">Read more!</a> </p> | ||
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+ | <table id="Fig8" class="imgtxt" style="margin-top:-41px; margin-right:-10px;"> | ||
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+ | <td> <a href="https://2013.igem.org/Team:Freiburg/Project/induction#light"> <img src="https://static.igem.org/mediawiki/2013/b/bc/Light-Freiburg-2013-Highlight-Boston.png"> </a> </td> | ||
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Revision as of 22:38, 16 October 2013
HIGHLIGHTS