Team:Freiburg/Highlights
From 2013.igem.org
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+ | <p id="headline" > <a href="https://2013.igem.org/Team:Freiburg/Project/crrna"> <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png" style="height:65px; width:65px; margin-top:-34px; margin-left:-4px; "> </a> | ||
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+ | One of the greatest advantages of the CRISPR/Cas9 system is that only one protein is required for targeting of various DNA sequences. The only component which needs to be replaced is the CRISPR-RNA (crRNA). We therefore designed an RNA plasmid termed the <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna#rnaimer">RNAimer</a>. It provides the backbone for easily exchanging the sequence for these crRNAs. Functional tests showed that the RNAimer plasmid works efficiently in mammalian cells. For multiple targeting, different crRNAs can be combined into one RNAimer plasmid. Gene regulation worked even more efficiently when using multiple targets. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna">Read more!</a> </p> | ||
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+ | <table id="Fig8" class="imgtxt" style="margin-top:111px; margin-right:-10px;"> | ||
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+ | <td> <a href="https://2013.igem.org/Team:Freiburg/Project/crrna#rnaimer"> <img style="width:580px; margin-left:-20px; margin-top:20px;" src="https://static.igem.org/mediawiki/2013/c/c6/Multiple_Targeting_Freiburg_2013.png"> </a> </td> | ||
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<div id="left_column"> | <div id="left_column"> | ||
- | <p id="headline" > <a href="https://2013.igem.org/Team:Freiburg/Project/ | + | <p id="headline" > <a href="https://2013.igem.org/Team:Freiburg/Project/induction#light"> <img src="https://static.igem.org/mediawiki/2013/9/9a/Freiburg_2013_main2_Licht.png" style="height:65px; width:65px; margin-top:-34px; margin-left:-4px; "> </a> |
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- | + | We aimed to control our system with light to allow gene regulation with high spatiotemporal resolution. We engineered a system for induction by red, UVB and blue light. The blue light system is based on the light-triggered interaction of CRY2 and CIB1. CRY2 was fused to dCas9 which - upon light-stimulus - can recruit the CIB1-VP16 fusion protein to any DNA sequence of interest. A 5-fold upregulation of SEAP reporter was achieved (*, p<0.05). dCas9-CRY2 was targeted simultaneously to four DNA sites upstream of the promoter. Numbers characterizing the crRNAs represent the distance from the translation start site. | |
+ | <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/induction#light">Read more!</a> </p> | ||
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- | <td> <a href="https://2013.igem.org/Team:Freiburg/Project/ | + | <td> <a href="https://2013.igem.org/Team:Freiburg/Project/induction#light"> <img src="https://static.igem.org/mediawiki/2013/b/bc/Light-Freiburg-2013-Highlight-Boston.png"> </a> </td> |
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<!-- Manual --> | <!-- Manual --> |
Revision as of 14:07, 19 October 2013
HIGHLIGHTS