Team:Freiburg/Highlights
From 2013.igem.org
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<p id="h4" style="color:#ffcc00; font-style:italic; font-size:24px; text-align:center">To summarize - In the last months we were able to ...</p> | <p id="h4" style="color:#ffcc00; font-style:italic; font-size:24px; text-align:center">To summarize - In the last months we were able to ...</p> | ||
- | < | + | <ul style="font-size:18px; margin-left:20px;"> |
- | <li>construct a catalytically inactive version of Cas9 (dCas9) and thus generate a DNA binding protein.</li> | + | <li> construct a catalytically inactive version of Cas9 (dCas9) and thus generate a DNA binding protein.</li> |
- | <li> | + | <li> make our <a id="link" href="http://parts.igem.org/Part:BBa_K1150000">dCas9</a> accessible to the whole iGEM community by mutating illegal iGEM restriction sites.</li> |
- | <li> | + | <li> combine this modified dCas9 with different transcriptional <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/effector">effectors</a>.</li> |
- | <li> | + | <li> express these fusion proteins in various mammalian cell lines.</li> |
- | <li> | + | <li> control mammalian gene expression via our modified CRISPR/Cas9 fusion proteins.</li> |
- | <li> | + | <li> build devices for controlling gene expression by different <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/induction#light">light stimuli</a>.</span> |
- | <li> | + | <li> provide an RNA plasmid termed <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna#rnaimer">RNAimer</a> for facile insertion of crRNAs which target desired target sequences.</li> |
- | <li> | + | <li> build an online tool that generates customized <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/toolkit">manuals</a> for easy usage of our toolkit.</li> |
- | <li> | + | <li> develop a method to assess the DNA binding capacity of our dCas9 fusion proteins - the <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/method#elisa">uniBAss</a>.</li> |
- | <li> | + | <li> <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/modeling">model</a> our system and obtain a possible insight into behavior of for measured and unmeasured components.</li> |
- | <li> | + | <li> truncate dCas9 and express different protein versions.</li> |
- | <li> | + | <li> build a cheap and functioning light box for light experiments calles "uniBOX".</li> |
</ul> | </ul> | ||
<p id="h4" style="color:#ffcc00; margin: none; text-align:center; font-style:italic; font-size:24px; margin-left:-43px">... design, develop, test and now offer a universally applicable toolkit for efficient gene <br> | <p id="h4" style="color:#ffcc00; margin: none; text-align:center; font-style:italic; font-size:24px; margin-left:-43px">... design, develop, test and now offer a universally applicable toolkit for efficient gene <br> | ||
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</div> | </div> | ||
</div> | </div> |
Revision as of 03:32, 28 October 2013
HIGHLIGHTS