Team:Freiburg/Highlights/2
From 2013.igem.org
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+ | Activation | ||
+ | </p> | ||
+ | <p> | ||
+ | |||
+ | For activation we choosed VP-16 as effector. Through the transactivating function of VP-16 the expression of these genes will be enhanced.<br> | ||
+ | We achieved up to 30-fold activation. | ||
+ | </p> | ||
+ | |||
+ | </div> | ||
+ | |||
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- | <div class=" | + | <div> |
- | + | <table id="Fig8" class="imgtxt"> | |
- | + | <tbody><tr> | |
- | + | <td> <img style="width:500px" src="https://static.igem.org/mediawiki/2013/5/5b/Freiburg2013-Highlights-VP16.png"> </td> | |
- | + | </tr> | |
- | + | <tr> | |
+ | <td style="height:10px"> <b>Figure 1:</b> Different error values plotted in increasing order.<br> </td> | ||
+ | </tr> | ||
+ | </tbody></table> | ||
+ | </div> | ||
+ | |||
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+ | <!-- <img src="https://static.igem.org/mediawiki/2013/2/20/Freiburg2013-Highlights-KRAB3.png" style="width:500px"> --> | ||
+ | |||
+ | <div> | ||
+ | <table id="Fig8" class="imgtxt"> | ||
+ | <tbody><tr> | ||
+ | <td> <img style="width:500px" src="https://static.igem.org/mediawiki/2013/2/20/Freiburg2013-Highlights-KRAB3.png"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="height:10px"> <b>Figure 2:</b> Different error values plotted in increasing order.<br> </td> | ||
+ | </tr> | ||
+ | </tbody></table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
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- | + | <p id="headline"> | |
- | + | Repression | |
- | + | </p> | |
- | + | <p> | |
- | + | The fusion of the transcriptional repressor domain KRAB leads to synthetic repression of gene expression. With this construct a strong repression could be observed. | |
+ | </p> | ||
+ | |||
+ | </div> | ||
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+ | Chromatin modification (Repression) | ||
+ | </p> | ||
+ | <p > | ||
+ | Specific chromatin modification was achieved by fusing the histone methyltransferase G9a to dCas9. With this protein we are able to specifically repress endogenous gene expression. | ||
+ | </p> | ||
+ | |||
+ | </div> | ||
+ | |||
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- | + | ||
- | + | ||
- | + | <!-- <img style="width:500px" src="https://static.igem.org/mediawiki/2013/6/6d/Freiburg2013-Highlights-Epigenetik2.png"> --> | |
- | + | ||
- | + | <div> | |
+ | <table id="Fig8" class="imgtxt"> | ||
+ | <tbody><tr> | ||
+ | <td> <img style="width:500px" src="https://static.igem.org/mediawiki/2013/6/6d/Freiburg2013-Highlights-Epigenetik2.png"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="height:10px"> <b>Figure 3:</b> Different error values plotted in increasing order.<br> </td> | ||
+ | </tr> | ||
+ | </tbody></table> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
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- | <img src="https://static.igem.org/mediawiki/2013/ | + | <div id="left_column"> |
+ | |||
+ | |||
+ | |||
+ | <div> | ||
+ | <table id="Fig8" class="imgtxt"> | ||
+ | <tbody><tr> | ||
+ | <td> <img style="width:500px" src="https://static.igem.org/mediawiki/2013/6/6b/Freiburg2013-Highlights-Multiple2.png"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="height:10px"> <b>Figure 4:</b> Different error values plotted in increasing order.<br> </td> | ||
+ | </tr> | ||
+ | </tbody></table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
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- | + | <p id="headline" > | |
- | + | Multiple Targeting | |
- | + | </p> | |
- | + | <p> | |
+ | One of the biggest advantages of the CRISPR/Cas9 system is that only one protein is required for targeting several DNA sites: For a new target there has to be just another guiding RNA. We designed an RNA plasmid, "<a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna#multiple_targeting">RNAimer</a>", containing this RNA. For multiple targeting different RNAimers can be easily combined using the iGEM BioBrick system.<br><br> | ||
+ | And as the results show, multiple targeting is possible and even better! | ||
+ | </p> | ||
+ | |||
+ | </div> | ||
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+ | uniBAss | ||
+ | </p> | ||
+ | <p> | ||
+ | We developed a novel and innovative ELISA based method to quantify the binding efficiency of our proteins. We called this binding assay uniBAss. This is a powerful tool for characterizing the modified dCas9 by assessing its DNA binding capacity with high throughput capabilities. | ||
+ | </p> | ||
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+ | </div> | ||
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- | + | <!--<img src="https://static.igem.org/mediawiki/2013/f/f6/UniBASS_Freiburg_2013.JPG" style="width:500px">--> | |
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+ | <table class="imgtxt" width="500px"> | ||
+ | <tr> | ||
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+ | <EMBED HEIGHT=362 WIDTH=400 TYPE="video/quicktime" | ||
+ | PLUGINSPAGE="http://www.apple.com/quicktime/download/" | ||
+ | SRC="https://static.igem.org/mediawiki/2013/a/a1/Freiburg_2013_highlights_UniBAss_400.mov" | ||
+ | AUTOPLAY="false"/></OBJECT> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="height:30px;"> <b>Figure 5: uniBAss - universal Binding Assay</b><br> | ||
+ | First step ... | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
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+ | </div> | ||
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Revision as of 11:24, 4 October 2013
HIGHLIGHTS