Team:Freiburg/Highlights/2

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HIGHLIGHTS

  • In the last months we were able to ...

    • ... construct a catalytically inactive version of Cas9 and thus generate a DNA binding protein.
    • ... combine this modified dCas9 with different transcriptional effectors.
    • ... express this fusion proteins in various mammalian cell lines.
    • ... control mammalian gene expression via our modified CRISPR/Cas fusion proteins.
    • ... build devices for controling gene expression by light stimulus.
    • ... provide an RNA plasmid for easily inserting sequences for crRNAs which target every desired target.
    • ... build an online tool that generates customized manuals for using our toolkit
    • ... develop a method to assess the DNA binding capacity of our dCas9-fusion proteins.
    • ... make our dCas9 accessible to the whole iGEM community by mutating illegal iGEM restriction sites.
    • ... In summary, we can now offer a universally applicable toolkit for gene regulation.

  • 6 opportunities with our uniCAS toolkit

    We provide 3 different effectors, 2 methods & 1 effector controller! Using our toolkit it's possible to efficiently activate or repress genes. We also provide devices for effector controling by light. Use our custom-tailored Manual Tool to generate your individual manual for your needs of gene regulation. Further it's possible to target not only one, but multiple genes of interest! And we established uniBAss - our universal Binding Assay. Best of all: It's open source and in iGEM standard!

  • dCas9 - The Heart of our toolkit

    We started by mutating the DNA cleavage site in the Cas9 protein and generated a sequence specific DNA binding protein that is relying on a protein-RNA-DNA interaction. We are now able to influence the DNA binding locus and can direct the protein to requested DNA targets.
    This is the heart of our toolkit. A protein that allows multiple and sequence specifid DNA binding.

    For simple and flexible regulation we do need now effectors, that can be fused to the protein.

  • The Toolbox

    Click here to read more about what the toolbox is and how you can use it.

  • The Toolbox

    Click here to read more about what the toolbox is and how you can use it.

  • The Toolbox

    Click here to read more about what the toolbox is and how you can use it.

  • The Toolbox

    Click here to read more about what the toolbox is and how you can use it.

  • The Toolbox

    Click here to read more about what the toolbox is and how you can use it.

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