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Team:Freiburg/Notebook/modeling
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<img src="https://static.igem.org/mediawiki/2013/8/88/Transfektionschema20.8.png" width="600px"> | <img src="https://static.igem.org/mediawiki/2013/8/88/Transfektionschema20.8.png" width="600px"> | ||
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To generate time dependent data, one well was treated every 6h.<br><br> | To generate time dependent data, one well was treated every 6h.<br><br> | ||
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t5= 07:50<br> | t5= 07:50<br> | ||
t6= 13:30<br> | t6= 13:30<br> | ||
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| + | Every timepoint the supernant was taken for SEAP measurement and the cells were lysated for quantitative western blotting. | ||
</div> | </div> | ||
Revision as of 09:10, 2 October 2013
Modeling Notebook
65000 cells per well were seeded. 24h before transfection.
The cells had been transfected at 21:30.
To generate time dependent data, one well was treated every 6h.
t0= 01:30
t1= 07:30
t2= 13:30
t3= 19:30
t4= 01:30
t5= 07:50
t6= 13:30
Every timepoint the supernant was taken for SEAP measurement and the cells were lysated for quantitative western blotting.
