Team:Hong Kong HKUST/data

From 2013.igem.org

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<br><br><br><div id="slide"><center><h3 class="title">Data Page</h3></center><br>  
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<h3>Introduction</h3>
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<p id="yo">For one of our human practice projects, our team investigated the current development of synthetic biology in some of the Asian countries. While doing research, we found out that synthetic biology is really a new field in science and that is not well-known in Asia. In fact, when some of our members tried to research information about synthetic biology in Cantonese, we could not find any webpages dedicated to share information about synthetic biology for the public in Hong Kong. To address this observation, we have decided to use an easily accessible platform called Google Blogger. This blog will be used as mean to introduce synthetic biology, share our experience and our project, and report current events related to synthetic biology. It will be written in Chinese and English so that most people in Hong Kong can read it easily and pay more attention to this new field in science. By providing holistic and informational blog posts, we aim at raising the public awareness to synthetic biology.</p><br>
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<p>This page shows all the parts we have made or used in our whole project. Click on the link below to see our constructs on the Registry of Biological Standard Part in detail. Click on main result page for an overview of our main result for those parts.<br>
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<h3>A Sneak Peek of Our Blog</h3><br>
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<center><img src="https://static.igem.org/mediawiki/2013/2/2e/Blog-cecilia.png" style="width:90%"></center><br>
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  <strong><br>
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  How our system works:</strong><br>
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  <strong>Data for Our Favorite New Parts:</strong>
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  <li><strong>Mitochondria leader sequence, BBa_K1119001</strong> : for directing protein into mitochondria</li>
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  <li>In the presence of mitochondria leader sequence, glyoxylate enzymes AceA and AceB can be led into mitochondria to introduce glyoxylate shunt.</li>
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  <li><strong>Cytomegalovirus promoter (CMV), BBa_K1119006</strong>: constitutive mammalian promoter</li>
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  <li>With the pCMV, glyoxylate genes AceA and AceB can be driven to be expressed.</li>
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<p id="hey">CLICK <a href="http://synbiohk.blogspot.hk">HERE</a> TO VISIT OUR BLOG! </p>
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    <strong>Data for Pre-existing Parts:</strong>
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      <li>strong>Kozak-XbaI-NLS-6His-stop, BBa_J176171</strong> (Haynes Lab, 2007):  used as a backbone for pfadBA and FABP.</li>
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      <li><strong>hGH-PolyA, </strong><strong>BBa_K404108</strong></a><strong> </strong>(Freiburg Bioware iGEM, 2010):  PolyA tail was added after the GFP sequence in the construct of <strong>BBa_K1119008 </strong>and<strong> BBa_K1119009 </strong>for characterization of MLS, which gave us the expected results.</li>
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      <li><strong>GFP BBa_K648013</strong>(Penn-State iGEM, 2011): green fluorescent protein reporter (E0040) with standard 25 prefix and suffix was used as a reporter for characterization of MLS and pCMV.</li>
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We've Also Characterized the Following Parts</strong><br>
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    <li><strong>CMV regulated GFP, BBa_K1119008: </strong>By assembling pCMV with GFP-PolyA, and transfection into HEK293FT cells, GFP can be successfully expressed.</li>
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    <li><strong>Mitochondria Leader Sequence – CMV promoter, BBa_K1119009</strong>: By assembling pCMV with MLS and GFP-PolyA, GFP can be successfully expressed and directed into mitochondria after transfection into HEK293FT cells.</li>
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  </ul>
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Revision as of 15:23, 27 September 2013




Data Page


This page shows all the parts we have made or used in our whole project. Click on the link below to see our constructs on the Registry of Biological Standard Part in detail. Click on main result page for an overview of our main result for those parts.


How our system works:


Data for Our Favorite New Parts:

  • Mitochondria leader sequence, BBa_K1119001 : for directing protein into mitochondria
  • In the presence of mitochondria leader sequence, glyoxylate enzymes AceA and AceB can be led into mitochondria to introduce glyoxylate shunt.
  • Cytomegalovirus promoter (CMV), BBa_K1119006: constitutive mammalian promoter
  • With the pCMV, glyoxylate genes AceA and AceB can be driven to be expressed.


Data for Pre-existing Parts:
  • strong>Kozak-XbaI-NLS-6His-stop, BBa_J176171 (Haynes Lab, 2007):  used as a backbone for pfadBA and FABP.
  • hGH-PolyA, BBa_K404108 (Freiburg Bioware iGEM, 2010):  PolyA tail was added after the GFP sequence in the construct of BBa_K1119008 and BBa_K1119009 for characterization of MLS, which gave us the expected results.
  • GFP BBa_K648013(Penn-State iGEM, 2011): green fluorescent protein reporter (E0040) with standard 25 prefix and suffix was used as a reporter for characterization of MLS and pCMV.


We've Also Characterized the Following Parts
  • CMV regulated GFP, BBa_K1119008: By assembling pCMV with GFP-PolyA, and transfection into HEK293FT cells, GFP can be successfully expressed.
  • Mitochondria Leader Sequence – CMV promoter, BBa_K1119009: By assembling pCMV with MLS and GFP-PolyA, GFP can be successfully expressed and directed into mitochondria after transfection into HEK293FT cells.

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