BBa_J176171

BBa_ J176171 was planned to be used as a mammalian vector backbone for the promoters. After running PCR (Polymerase Chain Reaction) to amplify the FABP1 promoter and ligated it with GFP and the backbone, we did mutagenesis for the vector. Based on the gel photo, we concluded that the vector was easy to get degraded. Finally we figured out that BBa-J176171 was heat unstable and changed our plan.