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Protein Trafficking
Responsible for:
Target ACE proteins into mitochondria
Parts submitted: BBa_K1119000, BBa_K1119001 & BBa_K1119009
Team:Hong Kong HKUST/modules
From 2013.igem.org
Project Outline
The design of our project is to build a constitutive and inducible glyoxylate shunt that increases energy metabolism by accelerating fatty acid uptake rate. First, the glyxylate shunt is introduced into mammalian cell by two bacterial native genes, aceA and aceB. However, unlike bacteria, citric acid cycle occurs in mitochondria for mammals. Thus, we translocated glyoxylate enzymes into mitochondria by fusing them with mitochondrial leader sequence. Lastly, for the glyoxylate enzymes to be expressed constitutively, we have fused them with mammalian constitutive promoters, namely CMV and EF-1alpha promoters. For inducible circuit, the glyoxylate enzymes are designed to be fused with fatty acid inducible promoters such as fatty acid binding protein promoter. Hover your mouse and click on the images below to learn more about each modules!
Cell Viability & Fatty Acid Quantification
Responsible for:
Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate
Parts submitted: -
Fatty Acid Sensing Mechanism
Responsible for:
Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration
Parts submitted: -
Glyoxylate Shunt
Responsible for:
Introduce glyoxylate enzymes responsible for the shunt
Parts submitted:BBa_K1119002, BBa_K1119003, BBa_K1119004, BBa_K1119006 & BBa_K1119008
