Team:INSA Toulouse

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Let's count with ''<i>E. Calculus</i>''</div>

Let's count with ''<i>E. Calculus</i>''</div>

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Transposition of Boolean operators into genetic devices is one of the various goals of Synthetic Biology. There are many difficulties associated with the biological use of Genetic Boolean Operators (GBO) the major being their inherent inability to make a clear difference between the 0 and 1 state, usually because the 0 state is rarely equal to no response in biological systems. Our project will try to tackle this problem, taking advantage of the most recent technology breakthrough in the design of GBO with the use of recombinases as an entry signal to promote the change of state required in the logical gate. A clear advantage of recombinases is that these protein are active at very small concentrations and are therefore very little sensitive to expression variations within a population. Secondly, being a genetic event, recombination will effectively define the two distinct states (0 or 1) required by the logical gate: recombined or not recombined.  

Transposition of Boolean operators into genetic devices is one of the various goals of Synthetic Biology. There are many difficulties associated with the biological use of Genetic Boolean Operators (GBO) the major being their inherent inability to make a clear difference between the 0 and 1 state, usually because the 0 state is rarely equal to no response in biological systems. Our project will try to tackle this problem, taking advantage of the most recent technology breakthrough in the design of GBO with the use of recombinases as an entry signal to promote the change of state required in the logical gate. A clear advantage of recombinases is that these protein are active at very small concentrations and are therefore very little sensitive to expression variations within a population. Secondly, being a genetic event, recombination will effectively define the two distinct states (0 or 1) required by the logical gate: recombined or not recombined.  

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To demonstrate the validity of these two state logic gates, we will create an n-bits full-adder using recombinases-based AND and XOR logical gates. The <i>E. calculus</i> strain should be able to execute full n-bits counting, taking in account the carry at each stage. These systems should approach as much possible the reliability of an electronic two digit device and help the Synthetic Biology community building strong and robust GBOs.

To demonstrate the validity of these two state logic gates, we will create an n-bits full-adder using recombinases-based AND and XOR logical gates. The <i>E. calculus</i> strain should be able to execute full n-bits counting, taking in account the carry at each stage. These systems should approach as much possible the reliability of an electronic two digit device and help the Synthetic Biology community building strong and robust GBOs.

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