Biological Construction

Riboregulation System

In order to better control the expression of the recombinases, a ribo regulation system has been add before the recombinases genes.

How does it work ?

This is the basic system : Two promoters each followed by a RNA sequence

The blue and the red regions are regions which code for little RNA sequences.


When P2 is activated and not P1, the red sequence will be transcripted. This red sequence possess a similarity with the rbs sequence. So when transcripted the red sequence will confine the rbs and prevent the recombinase from being translated.

When P1 and P2 are activated, both sequences (the blue and the red one) are transcripted.
The blue RNA sequence possess a stronger similiraty with the red one. When transcripted the blue and the red sequences will match together and release the rbs to permit the translation of the recombinase.

Our strategy

These publications "Genetic switchboard for synthetic biology applications" and "Engineered riboregulators enable post-transcriptional control of gene expression"inspired us. In their work they present this riboregulation system and gave the sequences of the blue and the red RNA, we choose to design 5 others riboswitches keeping some sequences from the original one and changing some nucleotides.