Revision as of 18:28, 21 September 2013

BioBricks

<groupparts>iGEM013 Imperial_College</groupparts>

Data

Glucose growth assay

Cell growth of phaABC E. coli at 4 concentrations of glucose. Optimum growth is at 2-4% glucose at 37ºC. Error bars represents SE of the mean, n=4

L-lactic Acid growth assay

Cell growth of MG1655 on 5mM L-Lactic Acid. Error bars represents SE of the mean, n=4.

WCM growth and IPTG induction assay Originally we intended on using [http://parts.igem.org/Part:BBa_K639003 BBa_K639003] to detect whether our cells were stressed when placed in various toxic byproducts. However, as the data below shows, this biobrick is very leaky. As such, we are using the stress sensor as a marker for cell growth and also to show that the cells had been successfully transformed with the correct chloramphenicol resistance.

Cell growth over 6h with IPTG induction. mCherry production is induced by the stress pathway and detection of ppGpps. In order to bypass this, we induced with IPTG which inhibits LacI, resulting in mCherry expression.	Fluorescence of the cells under IPTG induction over a 6h period.

Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC. Error bars represents SE of the mean, n=4	Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated WCM for 48 hours.

(A) WCM precursor material, this sterilised media made from LB and SRF was used to produce all WCM utilised. (B) Cells containing mCherry pigment grown in SRF (A) over 3 days, then streaked in a qualitative assay to check for growth. (C) mCherry cells were streaked again after 7 days growth in SRF.

(A) SRF in PBS (phosphate buffered saline), a buffer. We can see from this experiment whether our bacteria can grow solely on the waste SRF. (B) Cells containing mCherry pigment grown in SRF (A) over 3 days, then streaked in a qualitative assay to check for growth. (C) mCherry cells were streaked again after 6 days growth in SRF.

Ethylene glycol growth assay

Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0mM, 100mM or 200mM Ethylene Glycol at 30ºC. Error bars represents SE of the mean, n=4

Our Sponsors

125px

@@ Line 10: / Line 10: @@
 <h1>Data</h1>
-<b>Glucose growth assay</b>
+<h2>Glucose growth assay</h2>
 [[File:Glucose_graph.png|thumbnail|center|600px|Cell growth of phaABC <i>E. coli</i> at 4 concentrations of glucose. Optimum growth is at 2-4% glucose at 37ºC. Error bars represents SE of the mean, n=4]]
 <br> <br>
-<b>L-lactic Acid growth assay</b>
+<h2>L-lactic Acid growth assay</h2>
 [[File:L-LActic_Acid.png|thumbnail|center|600px|Cell growth of MG1655 on 5mM L-Lactic Acid. Error bars represents SE of the mean, n=4.]]
 <br> <br>
@@ Line 37: / Line 37: @@
 <br> <br>
-<b>Ethylene glycol growth assay</b>
+<h2>Ethylene glycol growth assay</h2>
 [[File:EG_growth.png|thumbnail|center|600px|Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0mM, 100mM or 200mM Ethylene Glycol at 30ºC. Error bars represents SE of the mean, n=4]]

Team:Imperial College/data

From 2013.igem.org