Team:Imperial College/data
From 2013.igem.org
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<h1>Western blots</h1> | <h1>Western blots</h1> | ||
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https://fbcdn-sphotos-e-a.akamaihd.net/hphotos-ak-ash3/1239511_525756247509606_2126777043_n.jpg | https://fbcdn-sphotos-e-a.akamaihd.net/hphotos-ak-ash3/1239511_525756247509606_2126777043_n.jpg | ||
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<h1>Enzyme Kinetics</h1> | <h1>Enzyme Kinetics</h1> | ||
<h1>PHB production</h1> | <h1>PHB production</h1> | ||
- | + | [[File:Waste_cocktail.png|thumbnail|center|600px|<b>(A)</b> WCM precursor material, this sterilised media made from LB and SRF was used to produce all WCM utilised. <b>(B)</b> Cells containing mCherry pigment grown in SRF <b>(A)</b> over 3 days, then streaked in a qualitative assay to check for growth. <b>(C)</b> mCherry cells were streaked again after 7 days growth in SRF.]] | |
+ | [[File:PBS_Plus_Waste.jpg|thumbnail|center|600px|<b>(A)</b> SRF in PBS (phosphate buffered saline), a buffer. We can see from this experiment whether our bacteria can grow solely on the waste SRF. <b>(B)</b> Cells containing mCherry pigment grown in SRF <b>(A)</b> over 3 days, then streaked in a qualitative assay to check for growth. <b>(C)</b> mCherry cells were streaked again after 6 days growth in SRF.]] | ||
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Revision as of 23:44, 21 September 2013
Contents |
BioBricks
<groupparts>iGEM013 Imperial_College</groupparts>
Growth Assays
Glucose growth assay
L-lactic Acid growth assay
WCM growth and IPTG induction assay
Originally we intended on using [http://parts.igem.org/Part:BBa_K639003 BBa_K639003] to detect whether our cells were stressed when placed in various toxic byproducts. However, as the data below shows, this biobrick is very leaky. As such, we are using the stress sensor as a marker for cell growth and also to show that the cells had been successfully transformed with the correct chloramphenicol resistance.