Team:Imperial College/data
From 2013.igem.org
(Difference between revisions)
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<h2>Product inhibition on growth</h2> | <h2>Product inhibition on growth</h2> | ||
- | < | + | <h4>L-lactic Acid </h4> |
[[File:L-LActic_Acid.png|thumbnail|center|600px|Cell growth of MG1655 on 5mM L-Lactic Acid. Error bars represents SE of the mean, n=4.]] | [[File:L-LActic_Acid.png|thumbnail|center|600px|Cell growth of MG1655 on 5mM L-Lactic Acid. Error bars represents SE of the mean, n=4.]] | ||
- | < | + | <h3>Ethylene glycol </h3> |
[[File:EG_growth.png|thumbnail|center|600px|Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0mM, 100mM or 200mM Ethylene Glycol at 30ºC. Error bars represents SE of the mean, n=4]] | [[File:EG_growth.png|thumbnail|center|600px|Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0mM, 100mM or 200mM Ethylene Glycol at 30ºC. Error bars represents SE of the mean, n=4]] | ||
- | < | + | <h3>3-hydroxybutyrate (3HB) </h3> |
[[File:3HB666.png]] | [[File:3HB666.png]] | ||
- | < | + | <h3>Acetoacetate </h3> |
[[File:AA777.png]] | [[File:AA777.png]] | ||
- | < | + | <h3>Poly(3-hydroxybutyrate) P(3HB) </h3> |
[[File:31ug_P3HB.png]] | [[File:31ug_P3HB.png]] | ||
- | < | + | <h3>Poly(lactic acid) (PLA) </h3> |
[[File:PLA.png]] | [[File:PLA.png]] | ||
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<br> | <br> | ||
- | < | + | <h3>WCM growth and IPTG induction assay</h3> |
Originally we intended on using [http://parts.igem.org/Part:BBa_K639003 BBa_K639003] to detect whether our cells were stressed when placed in various toxic byproducts. However, as the data below shows, this biobrick is very leaky. As such, we are using the stress sensor as a marker for cell growth and also to show that the cells had been successfully transformed with the correct chloramphenicol resistance. | Originally we intended on using [http://parts.igem.org/Part:BBa_K639003 BBa_K639003] to detect whether our cells were stressed when placed in various toxic byproducts. However, as the data below shows, this biobrick is very leaky. As such, we are using the stress sensor as a marker for cell growth and also to show that the cells had been successfully transformed with the correct chloramphenicol resistance. | ||
{| class="wikitable" style="margin: 1em auto 1em auto;" | {| class="wikitable" style="margin: 1em auto 1em auto;" | ||
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<h2>Sole carbon source</h2> | <h2>Sole carbon source</h2> | ||
- | < | + | <h3>3HB</h3> |
[[File:3HB_sole_carbon_source.png]] | [[File:3HB_sole_carbon_source.png]] | ||
- | < | + | <h3>Acetoacetate</h3> |
[[File:AA_sole_carbon_source.png]] | [[File:AA_sole_carbon_source.png]] | ||
Revision as of 00:11, 22 September 2013
Contents |
BioBricks
<groupparts>iGEM013 Imperial_College</groupparts>
Growth Assays
Media characterisation
pBAD characterisation
Glucose
Product inhibition on growth
L-lactic Acid
Ethylene glycol
3-hydroxybutyrate (3HB)
Acetoacetate
Poly(3-hydroxybutyrate) P(3HB)
Poly(lactic acid) (PLA)
WCM growth and IPTG induction assay
Originally we intended on using [http://parts.igem.org/Part:BBa_K639003 BBa_K639003] to detect whether our cells were stressed when placed in various toxic byproducts. However, as the data below shows, this biobrick is very leaky. As such, we are using the stress sensor as a marker for cell growth and also to show that the cells had been successfully transformed with the correct chloramphenicol resistance.
Sole carbon source
3HB
Acetoacetate
Induction
Empty Vector Control
Western blots
Enzyme Kinetics
PHB production