Team:Leeds/Notebook
From 2013.igem.org
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* ''Collected our poster for YSB 1.0'' | * ''Collected our poster for YSB 1.0'' | ||
* ''Practiced presentation for YSB 1.0'' | * ''Practiced presentation for YSB 1.0'' | ||
- | * ''Transformed components for | + | * ''Transformed components for [[Team:Leeds/Project#Biosystem 2|Biosystem 2]]'' |
* ''Met with Rose Bavage and Louise Crabtree to discuss outreach events'' | * ''Met with Rose Bavage and Louise Crabtree to discuss outreach events'' | ||
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* ''Paul starts hiking along Hadrian's Wall for charity!'' | * ''Paul starts hiking along Hadrian's Wall for charity!'' | ||
* ''We made an array of buffers to go along with our characterization experiments'' | * ''We made an array of buffers to go along with our characterization experiments'' | ||
- | * ''Undertook a PCR cleanup of | + | * ''Undertook a PCR cleanup of Biosystem 2 products'' |
* ''Gels ran to check the PCR cleanup was successful'' | * ''Gels ran to check the PCR cleanup was successful'' | ||
- | * ''Assembly PCR of | + | * ''Assembly PCR of [[Team:Leeds/Project#Biosystem 1|Biosystem 1]] and [[Team:Leeds/Project#Biosystem 2|2]] - Attempt 1!'' |
<br> | <br> | ||
<div style="color:darkgreen;font-size:14pt">'''6th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''6th August'''</div> | ||
- | * ''Day 2 of hiking for Paul, raising money for the Crohn's and Ulcerative Collitis Unit in the Leeds Teaching Hospitals Trust | + | * ''Day 2 of hiking for Paul, raising money for the [http://www.justgiving.com/Paul-Turner20 Crohn's and Ulcerative Collitis Unit in the Leeds Teaching Hospitals Trust]'' |
* ''We ran a gel of yesterdays PCR products'' | * ''We ran a gel of yesterdays PCR products'' | ||
- | * ''Assembly PCR of | + | * ''Assembly PCR of [[Team:Leeds/Project#Biosystem 1|Biosystem 1]] and [[Team:Leeds/Project#Biosystem 2|2]] - Attempt 2!'' |
* ''Plasmid prep of GFP producing bacteria and transformation into expression cells for use as controls in experiements'' | * ''Plasmid prep of GFP producing bacteria and transformation into expression cells for use as controls in experiements'' | ||
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* ''[[Team:Leeds/Modeling|Modelling]] discussion with [[Team:Manchester|Manchester]] and [[Team:Newcastle|Newcastle]] regarding modelling tutorial videos - Leeds will be producing one on Virtual Cell and integration of BNGL with VCell'' | * ''[[Team:Leeds/Modeling|Modelling]] discussion with [[Team:Manchester|Manchester]] and [[Team:Newcastle|Newcastle]] regarding modelling tutorial videos - Leeds will be producing one on Virtual Cell and integration of BNGL with VCell'' | ||
* ''Lab digests'' | * ''Lab digests'' | ||
- | * ''Construction of [[Team:Leeds/Project# | + | * ''Construction of [[Team:Leeds/Project#Biosystem 1|Biosystem 1]]'' |
- | * ''Constructed growth curves for our bacteria that contained our | + | * ''Constructed growth curves for our bacteria that contained our biosystem'' |
* ''A draft copy of ethics assessment form was produced'' | * ''A draft copy of ethics assessment form was produced'' | ||
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* ''Wiki formatting cleaned up, favicon enabled, compatibility issue with Chrome and Safari fixed'' | * ''Wiki formatting cleaned up, favicon enabled, compatibility issue with Chrome and Safari fixed'' | ||
* ''New graph uploaded to [[Team:Leeds/Results|Results]] section'' | * ''New graph uploaded to [[Team:Leeds/Results|Results]] section'' | ||
- | * ''Construction of [[Team:Leeds/Project# | + | * ''Construction of [[Team:Leeds/Project#Biosystem 1|Biosystem 1]] finished off, ready for characterisation studies'' |
* ''Awaiting Fluoro-AFM time for characterisation protocols'' | * ''Awaiting Fluoro-AFM time for characterisation protocols'' | ||
* ''Started using GraphPad to draw our graphs from labs'' | * ''Started using GraphPad to draw our graphs from labs'' | ||
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* ''We treated our beads to make them hydrophobic for our experiments'' | * ''We treated our beads to make them hydrophobic for our experiments'' | ||
* ''Made lots of buffers and reagents for our Nickel column experiment'' | * ''Made lots of buffers and reagents for our Nickel column experiment'' | ||
- | * ''Additional transformations of | + | * ''Additional transformations of [[Team:Leeds/Project#Biosystem 2|Biosystem 2]] and our ordered DNA were undertaken'' |
- | * '' | + | * ''[[Team:Leeds/Project#Biosystem 1|Biosystem 1]] was digested and run on a gel to check it was the correct length'' |
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<div style="color:darkgreen;font-size:14pt">'''15th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''15th August'''</div> | ||
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<div style="color:darkgreen;font-size:14pt">'''16th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''16th August'''</div> | ||
- | * ''Preliminary Fluoro-AFM to help with characterisation of [[Team:Leeds/Project# | + | * ''Preliminary Fluoro-AFM to help with characterisation of [[Team:Leeds/Project#Biosystem 1|Biosystem 1]]'' |
* ''Meeting with [[Team:Leeds/Team#Michael McPherson|Mike]] to discuss progress, current aims, learning outcomes and looking towards building on this next year'' | * ''Meeting with [[Team:Leeds/Team#Michael McPherson|Mike]] to discuss progress, current aims, learning outcomes and looking towards building on this next year'' | ||
- | * ''Ran an SDS-PAGE gel to find out whether | + | * ''Ran an SDS-PAGE gel to find out whether our isolation of the INP protein was successful or not'' |
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<div style="color:darkgreen;font-size:14pt">'''19th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''19th August'''</div> | ||
* ''Added direct links to full image page for graphs in [[Team:Leeds/Results|Lab Results]]'' | * ''Added direct links to full image page for graphs in [[Team:Leeds/Results|Lab Results]]'' | ||
- | * '' | + | * ''Set up some '''more''' PCR tubes to run to make more of BS2'' |
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<div style="color:darkgreen;font-size:14pt">'''20th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''20th August'''</div> | ||
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<div style="color:darkgreen;font-size:14pt">'''21st August'''</div> | <div style="color:darkgreen;font-size:14pt">'''21st August'''</div> | ||
- | * ''Made a start on the characterisation protocol for BS1, 60 tubes needing to be prepared | + | * ''Made a start on the characterisation protocol for BS1, 60 tubes needing to be prepared - a lot of pipette action!'' |
* ''Ran a Colony PCR!'' | * ''Ran a Colony PCR!'' | ||
* ''Digested and ligated BS1 into the submission plasmid, ready to be send off to iGEM headquarters'' | * ''Digested and ligated BS1 into the submission plasmid, ready to be send off to iGEM headquarters'' | ||
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<div style="color:darkgreen;font-size:14pt">'''22nd August'''</div> | <div style="color:darkgreen;font-size:14pt">'''22nd August'''</div> | ||
- | * ''Carried on with | + | * ''Carried on with [[Team:Leeds/Project#Biosystem 2|Biosystem 2]] characterisation'' |
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<div style="color:darkgreen;font-size:14pt">'''23rd August'''</div> | <div style="color:darkgreen;font-size:14pt">'''23rd August'''</div> | ||
- | * '' | + | * ''Had a meeting with [[Team:Leeds/Team#Luke Bawazeer|Luke]], [[Team:Leeds/Team#James Ross|James]] and [[Team:Leeds/Team#Michael McPherson|Mike]] about how the project is going'' |
- | * '' | + | * ''Began characterising [[Team:Leeds/Project|Biosystems]] 2 and 3'' |
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</div> | </div> | ||
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<div class="accordion-content"> | <div class="accordion-content"> | ||
<div style="color:darkgreen;font-size:14pt">'''26th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''26th August'''</div> | ||
- | * '' | + | * ''University closed for August Bank Holiday'' |
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<div style="color:darkgreen;font-size:14pt">'''27th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''27th August'''</div> | ||
- | * '' | + | * ''University closed for August Bank Holiday'' |
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<div style="color:darkgreen;font-size:14pt">'''28th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''28th August'''</div> | ||
- | * '' | + | * ''Tried to fix HTML5 video tag bugs with Safari... no success yet'' |
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<div style="color:darkgreen;font-size:14pt">'''29th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''29th August'''</div> | ||
- | * '' | + | * ''Got video tag working with IE9+, but cannot upload the mpeg4 video format required to allow viewing. Videos still can be seen at our [http://www.youtube.com/user/igemleeds Youtube] page'' |
+ | * ''Added video thumbnails to videos embedded on the [[Team:Leeds/Outreach|Outreach]] page'' | ||
+ | * ''Prepped microscope slides for AFM'' | ||
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<div style="color:darkgreen;font-size:14pt">'''30th August'''</div> | <div style="color:darkgreen;font-size:14pt">'''30th August'''</div> | ||
- | * '' | + | * ''AFM scope slides coated and stored in walk-in fridge for the weekend'' |
+ | * ''Adjusted layout template for some new features on the wiki... coming soon!'' | ||
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</div></div> | </div></div> | ||
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<div class="accordion-content"> | <div class="accordion-content"> | ||
<div style="color:darkgreen;font-size:14pt">'''2nd September'''</div> | <div style="color:darkgreen;font-size:14pt">'''2nd September'''</div> | ||
- | * '' | + | * ''Began finding work-arounds to use javascript within MediaWiki framework'' |
+ | * ''Ran AFM of previously prepped slides. Culture does not seem to have taken very well. Refined slide preparation procedure'' | ||
+ | * ''Prepped fresh slides with grid pattern etc, then cleaned carefully to ensure optical perfection'' | ||
+ | * ''Potential work-around for javascript implemented, still some issues with certain script files'' | ||
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<div style="color:darkgreen;font-size:14pt">'''3rd September'''</div> | <div style="color:darkgreen;font-size:14pt">'''3rd September'''</div> | ||
- | * '' | + | * ''Fresh nights sleep led to new inspiration to fix javascript issues!'' |
+ | * ''All javascript files now uploaded as templates with instructions on use, within the iGEM wiki. Tested for bugs relating to this, all appear to work correctly'' | ||
+ | * ''All of wiki should now meet iGEM 2013 [https://2013.igem.org/Requirements/Wiki requirements], including all web2.0 elements. Just need to get video embeds working for IE9+ and Safari'' | ||
+ | * ''AFM slides cultured, and stored in walk in ready for Thursday'' | ||
<br> | <br> | ||
<div style="color:darkgreen;font-size:14pt">'''4th September'''</div> | <div style="color:darkgreen;font-size:14pt">'''4th September'''</div> |
Revision as of 11:34, 3 September 2013
Here's our log, you can read about what we've been doing in the lab and the office. (We're working hard, promise)
June 2013Week 124th June
25th June
26th June
27th June
28th June
July 2013Week 21st July
2nd July
3rd July
4th July
5th July
Week 38th July
9th July
10th July
11th July
12th July
Week 415th July
16th July
17th July
18th July
19th July
Week 522nd July
23rd July
24th July
25th July
26th July
Week 629th July
30th July
31st July
August 2013Week 61st August
2nd August
Week 75th August
6th August
7th August
8th August
9th August
Week 812th August
13th August
14th August
15th August
16th August
Week 919th August
20th August
21st August
22nd August
23rd August
Week 1026th August
27th August
28th August
29th August
30th August
September 2013Week 112nd September
3rd September
4th September
5th September
6th September
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