|
|
| (15 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| | + | <html> |
| | + | <head profile="https://2013.igem.org/Team:Leeds/Parts"> |
| | + | <link rel="icon" |
| | + | type="image/png" |
| | + | href="https://static.igem.org/mediawiki/2013/4/4d/Leeds_favicon.png"> |
| | + | </head> |
| | + | </html> |
| | __NOTOC__ | | __NOTOC__ |
| - | {{Team:Leeds_layout|Header=Leeds' Registry Parts|content= | + | {{Team:Leeds_layout|HeaderParky=</div>[[File:Leeds_ParkyParts.png|150px|link=|frameless]]<div>|HeaderImage=[[File:Leeds_PartsHeader.png|link=|825px|frameless]]|Header=Leeds' Registry Parts|content= |
| - | ''Please check back here frequently for a full list of the parts we've developed, submitted to the registry and have had approved. We will also endeavour to include our characeterisation protocols, although we are currently collaborating with [[Team:Purdue|Purdue]] to decide exactly what these should be.''
| + | ==Parts Summary== |
| - | <br>
| + | * [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028000 Silica Binding Peptide (Si4):] A short amino acid sequence that binds silica with high affinity. |
| - | __TOC__
| + | * [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028002 Bio-system 1:] CpxR promoter controlling GFP expression. Will produce the green fluorescent protein following exposure to hydrophobic membrane stress. Extensive experimental data has been acquired about the response of the cpxR promoter to various forms of physical and chemical stresses, this can be found in the Device 1 section of the Lab Results page. |
| - | ==Currently Working On== | + | * [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028003 Bio-system 2:] INP.Si4 is produced constitutively with GFP production under control of CpxR promoter. The system is designed to bind with high affinity to silica macro-structures and produce a fluorescent response when it does. Flow cytometry, fluorescence confocal, and scanning electron microscopy data has been gathered to characterize this part. Problems with expression meant that the data is not as conclusive as predicted but indicates that Si4 is expressed in some cells. |
| - | * Bio-system 1: pCpxR promoter activates GFP expression. Will produce the green fluorescent protein on membrane stress. | + | |
| - | * Bio-system 2: Constitutive genes produce YFP and Ice nucleation protein with a silica binding domain at the c-terminus.
| + | |
| - | * Bio-system 3: integration of 1 and 2, INP Si4is constitutively produced but the fluorescent protein is under control of pCpxR promoter. When bound to silica beads will fluoresce | + | |
| - | * Also planning on having the silica binding peptide submitted as its own part for others to use for their own purposes.
| + | |
| | <br> | | <br> |
| | ==Submitted== | | ==Submitted== |
| | A full listing of all parts Leeds iGEM 2013 have submitted to the Registry, and their status. | | A full listing of all parts Leeds iGEM 2013 have submitted to the Registry, and their status. |
| | <br> | | <br> |
| - | ==Characterisation Protocols==
| + | <groupparts>iGEM013 Leeds</groupparts> |
| - | * Bio-system 1: Bacteria exposed to hydrophobic beads to induce membrane stress and fluorescence response is measured using a flourimeter.
| + | <br> |
| - | * Bio-system 2: Bacteria exposed to silica beads and washed. Control with no silica binding domain. Those that have bound will be visualised on the beads and those that have not will be seen in the elution.
| + | |
| - | * Bio-system 3: Expose the bacteria to silica beads will fluoresce when bound and not fluoresce when unbound. Measure the fluorescence with flourimeter.
| + | |
| - | * The Si4 domain will be characterised with bio-system 2 if binding occurs.
| + | |
| - | | + | |
| | <br> | | <br> |
| | }} | | }} |
| - | <br>
| |
| - | <br>
| |
| - | <div style="color:red;background:black;tfont-weight:bold;font-size:36pt;text-align:center;padding:19px"> Everything Below Here To Be Removed </div>
| |
| - | <br>
| |
| - | <br>
| |
| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** -->
| |
| - |
| |
| - | <html>
| |
| - | <div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
| |
| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
| |
| - | This is a template page. READ THESE INSTRUCTIONS.
| |
| - | </div>
| |
| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
| |
| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
| |
| - | </div>
| |
| - | <div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
| |
| - | You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.
| |
| - | </div>
| |
| - | </div>
| |
| - | </html>
| |
| - |
| |
| - | <!-- *** End of the alert box *** -->
| |
| - |
| |
| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
| |
| - | !align="center"|[[Team:Leeds|Home]]
| |
| - | !align="center"|[[Team:Leeds/Team|Team]]
| |
| - | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leeds Official Team Profile]
| |
| - | !align="center"|[[Team:Leeds/Project|Project]]
| |
| - | !align="center"|[[Team:Leeds/Parts|Parts Submitted to the Registry]]
| |
| - | !align="center"|[[Team:Leeds/Modeling|Modeling]]
| |
| - | !align="center"|[[Team:Leeds/Notebook|Notebook]]
| |
| - | !align="center"|[[Team:Leeds/Safety|Safety]]
| |
| - | !align="center"|[[Team:Leeds/Attributions|Attributions]]
| |
| - | |}
| |
| - |
| |
| - |
| |
| - |
| |
| - | An important aspect of the iGEM competition is the use and creation of standard biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
| |
| - |
| |
| - | Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
| |
| - |
| |
| - |
| |
| - | <groupparts>iGEM013 Leeds</groupparts>
| |
"
