Team:NYMU-Taipei/Experiment/Wet Lab

Data for Our Favorite Parts 1. A ‘’’ OxyR-induced Promoter’’’ BBa_K1104201(TrxC promotor) 2. A ‘’’Transcription Factor Activated by Oxidative Stress’’’ BBa_K1104200 (OxyR ) we apply a constitutive promoter to the OxyR coding sequence to overexpress the OxyR protein, which is expected to boost the sensitivity of our ROS-induced promotors. 3. An ‘’’antimicrobial peptide’’’ BBa_K1104200 (Defensin1 ) the mechanism which insects use to protect against the invasion of microbes.

Data for pre-Existing and Optimized Parts We successfully improved the biobrick part: ahpC promoter ahpC (K362001). Originally created by the KIT-Kyoto 2010 iGEM team by taking the upstream 1000bp of the AhpC gene, we improved the promoter by first mutating the PstI cutting site to make it conform to Assembly standard 10. We then designed specific primers to cut short the original sequence of AhpCp promoter to trim out unneeded sequences make it more efficient. More details about the design can be found on the Team:NYMU-Taipei/Project/Inhibition/Sensor Sensor page.

The supplementary characterization and the application of AhpC promoter has been added to the experience page of BBa_K1104203 (AhpCp1000).

The AhpCp1000 derived OxyR-induced promoters we created are as follows:

• BBa_K1104204 (AhpCp2D1 promoter)
• BBa_K1104205 (AhpCp2 promoter)
• BBa_K1104206 (AhpCpD1 promoter)
• BBa_K1104207 (AhpCp1 promoter)