Team:NYMU-Taipei/Project/Inhibition/Inhibition circuit

From 2013.igem.org

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===Circuit Regulation Testing===
===Circuit Regulation Testing===
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1. For pTet (R0040):
+
<br>1.For pTet (R0040):</br>
 +
  <p> pTet(R0040)+ E0840....(controll) </p>
 +
  <p> J23102+ RBS+ tetR+ Term+ pTet+ E0840 </p>
 +
  <p> J23102+ RBS+ lacI+ Term+ pTet+ E0840 </p>
 +
<br>2. For pLux/cI hybrid (R0065):</br>
 +
  <p> pLux/cI(R0065)+ E0840....(controll) </p>
 +
  <p> J23102+ RBS+ cI+ Term+ R0065+ E0840 </p>
 +
  <p> J23102+ RBS+ luxR+ RBS+ luxI+ Term+ R0065+ E0840 </p>
 +
  <p> J23102+ RBS+ luxR+ RBS+ luxI+ RBS+ cI+ Term+ R0065+ E0840</p>
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 +
===Our expectation===
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<br>1.</br>
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<p>(1)can see GFP under fluroscence </p>
 +
<p>(2)can not see GFP under fluroscence </p>
 +
<p>(3)can see GFP under fluroscence again</p>
 +
<br> Conclusion: tetR coding sequence has the ability to inhibit pTet.</br>
 +
 +
<br>2.</br>
 +
<p>(1)can not see GFP under fluroscence </p>
 +
<p>(2)can not see GFP under fluroscence </p>
 +
<p>(3)can see GFP under fluroscence </p>
 +
<p>(4)can not see GFP under fluroscence </p>
 +
<br> Conclusion: cI coding sequence is more dominant than LuxR/AHL, and cI coding sequence can well inhibit pLux/cI as well as LuxR& LuxI hibrid can initiate pLux/cI only when cI is absent.</br>
{{:Team:NYMU-Taipei/Footer}}
{{:Team:NYMU-Taipei/Footer}}

Revision as of 18:42, 28 October 2013

National Yang Ming University



Circuit Regulation Vedio

Circuit Regulation Testing


1.For pTet (R0040):</br>

pTet(R0040)+ E0840....(controll)

J23102+ RBS+ tetR+ Term+ pTet+ E0840

J23102+ RBS+ lacI+ Term+ pTet+ E0840


2. For pLux/cI hybrid (R0065):</br>

pLux/cI(R0065)+ E0840....(controll)

J23102+ RBS+ cI+ Term+ R0065+ E0840

J23102+ RBS+ luxR+ RBS+ luxI+ Term+ R0065+ E0840

J23102+ RBS+ luxR+ RBS+ luxI+ RBS+ cI+ Term+ R0065+ E0840

Our expectation


1.</br>

(1)can see GFP under fluroscence

(2)can not see GFP under fluroscence

(3)can see GFP under fluroscence again


Conclusion: tetR coding sequence has the ability to inhibit pTet.</br>


2.</br>

(1)can not see GFP under fluroscence

(2)can not see GFP under fluroscence

(3)can see GFP under fluroscence

(4)can not see GFP under fluroscence


Conclusion: cI coding sequence is more dominant than LuxR/AHL, and cI coding sequence can well inhibit pLux/cI as well as LuxR& LuxI hibrid can initiate pLux/cI only when cI is absent.</br>