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Team:Paris Saclay/Notebook/August/13
From 2013.igem.org
Contents |
Notebook : August 13
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining ...
1 - Electrophoresis to chech the digestion of Bba_K1155000 by SpeI and PstI
Nadia
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Expected sizes :
- Pfnr : ...
- PSB1C3 : ...
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We can't see any band for Bba_K1155000 digestion. The digestion failed because we used the wrong enzymes. We will do it again with the good ones. |
2 - Digestion of Bba_K1155000, Bba_K1155004, Bba_K1155005, Bba_K1155006 by SpeI and by SpeI/EcoRI
Anaïs, Nadia
- Bba_K1155000 :
- Buffer : 5µL
- H2O : 39µL
- DNA : 5µL
- SpeI : 1µL
- Bba_K1155000 :
- Buffer : 5µL
- H2O : 38µL
- DNA : 5µL
- SpeI : 1µL
- EcoRI : 1µL
- Bba_K1155004, Bba_K1155005, bba_K1155006 :
- Buffer : 2µL
- H2O : 7µL
- DNA : 10µL
- SpeI : 1µL
- Bba_K1155004, Bba_K1155005, bba_K1155006 :
- Buffer : 2µL
- H2O : 6µL
- DNA : 10µL
- SpeI : 1µL
- EcoRI : 1µL
3 - Electrophoresis to check the digestion of Bba_K1155000, Bba_K1155004, Bba_K1155005, Bba_K1155006 by SpeI and by SpeI/EcoRI
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - PCR of FRN Part I, FNR Part II, BphR2 Part I
Anaïs
Protocol : 07/30/13
2 - Electrophoresis of PCR products : FRN Part I, FNR Part II, BphR2 Part I
Damir
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Expected sizes :
- FNR part I : 597 kb
- FNR part II : 200 kb
- BphR2 part I : 178 kb
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It's impossible to read the first gel. We do it again. In the second gel, we obtain FNR Part I and FNR Part II fragments at the right size. We can purify it. We also obtain BphR2 frangment at the right size but it was mix with other DNA frangments. We will try to make a gel purification of it. |
3 - Gel purification of PCR product : BphR2 Part I
Damir
