Team:Paris Saclay/Notebook/August/20

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=='''Lab work'''==
=='''Lab work'''==
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==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006'''====
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===='''1 - Sequences analysis'''====
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Damir, XiaoJing
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...
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===

Revision as of 14:16, 17 September 2013

Contents

Notebook : August 20

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006

1 - Sequences analysis

Damir, XiaoJing

...

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining FNR and BphR2 proteins

1 - PCR of BphR2 Part I

Damir

Used quantities :

PCR Program :

2 - Electrophoresis of PCR products : BphR2 Part I

Nadia

[[]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 40µL BphR2 PartI+8µl of 6X loading dye
  • Gel : 0.8%

We obtain a frangment at the right size. We can purify it.

2 - Gel purification of PCR products : BphR2 Part I

Damir, Nadia

Protocol : Gel purification

Nanodrop :

  • BphR2 Part I: .........

CONCLUSION ON A TOUS PERDU D'OU ON RECOMMENCE ENSUITE ??????