Team:Paris Saclay/Notebook/August/20

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=='''Lab work'''==
=='''Lab work'''==
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===''''''===
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==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006'''====
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===='''1 - '''====
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===='''1 - Sequences analysis'''====
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Damir, XiaoJing
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{|
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006.
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|}
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
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===='''Objective : obtaining FNR and BphR2 proteins'''====
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===='''1 - Electrophoresis of PCR products :  BphR2 Part I'''====
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Nadia
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{|
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| style="width:250px;border:1px solid black;" | [[File:Psgel2008.jpg|250px]]
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| style="width:350px;border:1px solid black;vertical-align:top;" |
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*Well 1 : 6µL DNA Ladder
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*Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye
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*Gel : 0.8%
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|}
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Expected sizes :
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* BphR2 Part I : 178bp
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{|
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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We obtained a frangment at the right size. We can purify it.
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|}
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====2 - Gel purification of PCR products : BphR2 Part I ====
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Damir, Nadia
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Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
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{|
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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We lost our fragment. We will do the PCR again.
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|}
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{| border="1" align="center"
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|[[Team:Paris Saclay/Notebook/August/19|<big>Previous day</big>]]
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|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
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|[[Team:Paris Saclay/Notebook/August/21|<big>Next day</big>]]
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|}
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 01:30, 5 October 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : August 20

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006

1 - Sequences analysis

Damir, XiaoJing

Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006.

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining FNR and BphR2 proteins

1 - Electrophoresis of PCR products : BphR2 Part I

Nadia

Psgel2008.jpg
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye
  • Gel : 0.8%

Expected sizes :

  • BphR2 Part I : 178bp

We obtained a frangment at the right size. We can purify it.

2 - Gel purification of PCR products : BphR2 Part I

Damir, Nadia

Protocol : Gel purification

We lost our fragment. We will do the PCR again.


Previous day Back to calendar Next day

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