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Team:Paris Saclay/Notebook/August/8
From 2013.igem.org
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=====Electrophoresis of BBa_J004450 digested by EcoRI/Pst1===== | =====Electrophoresis of BBa_J004450 digested by EcoRI/Pst1===== | ||
Damir, Nadia | Damir, Nadia | ||
| + | |||
| + | Goal : check if the digestion went right. | ||
'''Experiment''' | '''Experiment''' | ||
Revision as of 14:23, 9 August 2013
Contents |
Notebook : August 8
Lab work
Aerobic/Anaerobic regulation system
Preparation of the PSB3K3 backbone plasmid
Electrophoresis of BBa_J004450 digested by EcoRI/Pst1
Damir, Nadia
Goal : check if the digestion went right.
Experiment
| IMAGE |
Well 1 : 6µL DNA Ladder Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 Gel : 0.8% |
Analysis
Expected sizes :
PSB3K3 : 2750kb GFP : 1069kb
We obtained fragments of the right size.
